Projekt per år
Sammanfattning
Hydrogels play an important role in tissue engineering due to their native extracellular matrix-like characteristics, but they are insufficient in providing the necessary stimuli to support tissue formation. Efforts to integrate bioactive cues directly into hydrogels are hindered by incompatibility with hydrophobic drugs, issues of burst/uncontrolled release, and rapid degradation of the bioactive molecules. Skeletal muscle tissue repair requires internal stimuli and communication between cells for regeneration, and nanocomposite systems offer to improve the therapeutic effects in tissue regeneration. Here, the versatility of mesoporous silica nanoparticles (MSN) was leveraged to formulate a nanoparticle-hydrogel composite and to combine the benefits of controlled delivery of bioactive cues and cellular support. The tunable surface characteristics of MSNs were exploited to optimize homogeneity and intracellular drug delivery in a 3D matrix. Nanocomposite hydrogels formulated with acetylated or succinylated MSNs achieved high homogeneity in 3D distribution, with succinylated MSNs being rapidly internalized and acetylated MSNs exhibiting slower cellular uptake. MSN-hydrogel nanocomposites simultaneously allowed efficient local intracellular delivery of a hydrophobic model drug. To further study the efficiency of directing cell response, a Notch signaling inhibitor (DAPT) was incorporated into succinylated MSNs and incorporated into the hydrogel. MSN-hydrogel nanocomposites effectively downregulated the Notch signaling target genes, and accelerated and maintained the expression of myogenic markers. The current findings demonstrate a proof-of-concept in effective surface engineering strategies for MSN-based nanocomposites, suited for hydrophobic drug delivery in tissue regeneration with guided cues.
Originalspråk | Engelska |
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Artikelnummer | 100865 |
Antal sidor | 16 |
Tidskrift | Materials Today Bio |
Volym | 23 |
DOI | |
Status | Publicerad - dec. 2023 |
MoE-publikationstyp | A1 Tidskriftsartikel-refererad |
Fingeravtryck
Fördjupa i forskningsämnen för ”Directing cellular responses in a nanocomposite 3D matrix for tissue regeneration with nanoparticle-mediated drug delivery”. Tillsammans bildar de ett unikt fingeravtryck.Utrustning
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Åbo Akademi Functional Printing Center
Toivakka, M. (PI), Rosenholm, J. (PI), Anttu, N. (PI), Bobacka, J. (PI), Huynh, T. P. (PI), Peltonen, J. (PI), Wang, X. (PI), Wilen, C.-E. (PI), Xu, C. (PI), Zhang, H. (PI) & Österbacka, R. (PI)
Fakulteten för naturvetenskaper och teknikUtrustning/facilitet: Facilitet
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3D Printing for Personalized Medicine and Customized Drug Delivery
Viitala, T. (Ansvarig forskare), Rosenholm, J. (CoPI) & Xu, C. (CoPI)
01/05/23 → 30/04/25
Projekt: Företag/Business Finland
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AMBioPharma: Centre for Additive Manufacturing for Life Science and Pharmaceutical Industry
Rosenholm, J. (Ansvarig forskare), Xu, C. (Ansvarig forskare), Wang, X. (CoI) & Palo, M. (CoI)
01/09/21 → 31/08/23
Projekt: EU