Purification and Validation of Lipid Transfer Proteins

Matti Kjellberg, Anders Backman, Anna Möuts, Peter Mattjus

Forskningsoutput: Kapitel i bok/konferenshandlingKapitelVetenskapligPeer review

2 Citeringar (Scopus)

Sammanfattning

Understanding the holistic picture of lipid homeostasis involves not only the analysis of synthesis and breakdown of lipids, but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity.

OriginalspråkOdefinierat/okänt
Titel på gästpublikationLipidomics - Methods and Protocols
RedaktörerSanjoy K. Bhattacharya
FörlagHumana Press
Sidor
ISBN (tryckt)978-1-4939-6995-1
StatusPublicerad - 2017
MoE-publikationstypA3 Del av bok eller annan forskningsbok

Nyckelord

  • Fluorescence
  • lipid
  • surface plasmon resonance
  • vesicle

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