Purification and Validation of Lipid Transfer Proteins

Matti Kjellberg, Anders Backman, Anna Möuts, Peter Mattjus

Tutkimustuotos: Artikkeli kirjassa/raportissa/konferenssijulkaisussaLukuTieteellinenvertaisarvioitu

2 Sitaatiot (Scopus)

Abstrakti

Understanding the holistic picture of lipid homeostasis involves not only the analysis of synthesis and breakdown of lipids, but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity.

AlkuperäiskieliEi tiedossa
OtsikkoLipidomics - Methods and Protocols
ToimittajatSanjoy K. Bhattacharya
KustantajaHumana Press
Sivut
ISBN (painettu)978-1-4939-6995-1
TilaJulkaistu - 2017
OKM-julkaisutyyppiA3 Kirjan osa tai toinen tutkimuskirja

Keywords

  • Fluorescence
  • lipid
  • surface plasmon resonance
  • vesicle

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