Purification and Validation of Lipid Transfer Proteins

Matti Kjellberg, Anders Backman, Anna Möuts, Peter Mattjus

Research output: Chapter in Book/Conference proceedingChapterScientificpeer-review

2 Citations (Scopus)

Abstract

Understanding the holistic picture of lipid homeostasis involves not only the analysis of synthesis and breakdown of lipids, but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity.

Original languageUndefined/Unknown
Title of host publicationLipidomics - Methods and Protocols
EditorsSanjoy K. Bhattacharya
PublisherHumana Press
Pages
ISBN (Print)978-1-4939-6995-1
Publication statusPublished - 2017
MoE publication typeA3 Part of a book or another research book

Keywords

  • Fluorescence
  • lipid
  • surface plasmon resonance
  • vesicle

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