Sammanfattning
The paired-like homeobox transcription factor LEUTX is expressed in human preimplantation embryos between the 4- and 8-cell stages, and then silenced in somatic tissues. To characterize the function of LEUTX, we performed a multiomic characterization of LEUTX using two proteomics methods and three genome-wide sequencing approaches. Our results show that LEUTX stably interacts with the EP300 and CBP histone acetyltransferases through its 9 amino acid transactivation domain (9aaTAD), as mutation of this domain abolishes the interactions. LEUTX targets genomic cis-regulatory sequences that overlap with repetitive elements, and through these elements it is suggested to regulate the expression of its downstream genes. We find LEUTX to be a transcriptional activator, upregulating several genes linked to preimplantation development as well as 8-cell-like markers, such as DPPA3 and ZNF280A. Our results support a role for LEUTX in preimplantation development as an enhancer binding protein and as a potent transcriptional activator.
Originalspråk | Engelska |
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Artikelnummer | 106172 |
Antal sidor | 29 |
Tidskrift | iScience |
Volym | 26 |
Nummer | 3 |
DOI | |
Status | Publicerad - 17 mars 2023 |
MoE-publikationstyp | A1 Tidskriftsartikel-refererad |
Nyckelord
- Molecular biology
- Reproductive medicine
- Omics