@inbook{bfe79208ad904d408cff3f1bb99092b0,
title = "Isolation of the B Cell Immune Synapse for Proteomic Analysis",
abstract = "Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.",
keywords = "B cells, Cell adhesion, Immunological synapse immune synapse, Mass spectrometry, Proteomics",
author = "Cunha, {Diogo M.} and Sara Hern{\'a}ndez-P{\'e}rez and Mattila, {Pieta K.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
month = apr,
day = "28",
doi = "10.1007/978-1-0716-3135-5_25",
language = "English",
isbn = "978-1-0716-3134-8",
volume = "2654",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "393--408",
booktitle = "Methods in Molecular Biology",
}