Isolation of the B Cell Immune Synapse for Proteomic Analysis

Diogo M. Cunha, Sara Hernández-Pérez, Pieta K. Mattila*

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedingChapterScientificpeer-review

3 Citations (Scopus)

Abstract

Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages393-408
Number of pages16
Volume2654
ISBN (Print)978-1-0716-3134-8
DOIs
Publication statusPublished - 28 Apr 2023
MoE publication typeA3 Part of a book or another research book

Publication series

NameMethods in Molecular Biology
Volume2654
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • B cells
  • Cell adhesion
  • Immunological synapse immune synapse
  • Mass spectrometry
  • Proteomics

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