Abstract
Liquid chromatography-electrospray ionisation mass spectrometry was evaluated in the high-throughput analysis of microcystins and nodularins, cyanobacterial peptide hepatotoxins. Extracts originating from cyanobacterial strains and field material were separated on a 30 mm x 4 mm I.D. Merck Purospher STAR RP-18e column using a rapid gradient of aqueous formic acid and acetonitrile, ionised by electrospray technique and analysed on a Micromass Quattro II triple-quadrupole mass spectrometer operated in the selected ion recording (SIR) mode. The total analysis time per sample was 2.8 min corresponding to 514 samples a day. The system showed good robustness during a series of 320 repetitive injections of a field sample containing three major microcystins.
| Original language | English |
|---|---|
| Pages (from-to) | 291-298 |
| Number of pages | 8 |
| Journal | Chromatographia |
| Volume | 59 |
| Issue number | 5-6 |
| Publication status | Published - Mar 2004 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- Column liquid chromatography
- Cyanobacteria
- Mass spectrometry detection
- Microcystins and nodularins
- Toxins