High-throughput screening of ten microcystins and nodularins, cyanobacterial peptide hepatotoxins, by reversed-phase liquid chromatography-electrospray ionisation mass spectrometry

J. Meriluoto*, K. Karlsson, L. Spoof

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

40 Citations (Scopus)

Abstract

Liquid chromatography-electrospray ionisation mass spectrometry was evaluated in the high-throughput analysis of microcystins and nodularins, cyanobacterial peptide hepatotoxins. Extracts originating from cyanobacterial strains and field material were separated on a 30 mm x 4 mm I.D. Merck Purospher STAR RP-18e column using a rapid gradient of aqueous formic acid and acetonitrile, ionised by electrospray technique and analysed on a Micromass Quattro II triple-quadrupole mass spectrometer operated in the selected ion recording (SIR) mode. The total analysis time per sample was 2.8 min corresponding to 514 samples a day. The system showed good robustness during a series of 320 repetitive injections of a field sample containing three major microcystins.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalChromatographia
Volume59
Issue number5-6
Publication statusPublished - Mar 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • Column liquid chromatography
  • Cyanobacteria
  • Mass spectrometry detection
  • Microcystins and nodularins
  • Toxins

Fingerprint

Dive into the research topics of 'High-throughput screening of ten microcystins and nodularins, cyanobacterial peptide hepatotoxins, by reversed-phase liquid chromatography-electrospray ionisation mass spectrometry'. Together they form a unique fingerprint.

Cite this