Glycolipid transfer protein knockout disrupts vesicle trafficking to the plasma membrane

Henrik Nurmi, Anders Backman, Josefin Halin, Max Lönnfors, Tomas Blom, Pia Roos-Mattjus, Peter Mattjus*

*Korresponderande författare för detta arbete

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    2 Citeringar (Scopus)
    44 Nedladdningar (Pure)

    Sammanfattning

    The glycolipid transfer protein (GLTP) has been linked to many cellular processes aside from its best-known in vitro function as a lipid transport protein. It has been proposed to act as a sensor and regulator of glycosphingolipid homeostasis in cells. Furthermore, through its previously determined interaction with the endoplasmic reticulum membrane protein VAP-A (vesicle-associated membrane protein-associated protein A), GLTP may also be involved in facilitating vesicular transport in cells. In this study, we characterized the phenotype of CRISPR/Cas9-mediated GLTP KO HeLa cells. We showed that motility, three-dimensional growth, and cellular metabolism were all altered by GLTP knockout. Expression of a GLTP mutant incapable of binding VAP disrupted cell spheroid formation, indicating that the GLTP–VAP interaction is linked to cellular adhesion, cohesion, and three-dimensional growth. Most notably, we found evidence that GLTP, through its interaction with VAP-A, affects vesicular trafficking, marking the first cellular process discovered to be directly impacted by a change in GLTP expression.

    OriginalspråkEngelska
    Artikelnummer104607
    Antal sidor17
    TidskriftJournal of Biological Chemistry
    Volym299
    Nummer4
    DOI
    StatusPublicerad - apr. 2023
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Fingeravtryck

    Fördjupa i forskningsämnen för ”Glycolipid transfer protein knockout disrupts vesicle trafficking to the plasma membrane”. Tillsammans bildar de ett unikt fingeravtryck.

    Citera det här