Enzymatic Boolean Logic Gates toward ON/OFF Sensing

Paolo Bollella; Eleonora Macchia; Luisa Torsi

doi:10.1109/FLEPS53764.2022.9781600

Enzymatic Boolean Logic Gates toward ON/OFF Sensing

Paolo Bollella, Eleonora Macchia, Luisa Torsi

Physics

Research output: Chapter in Book/Conference proceeding › Conference contribution › Scientific › peer-review

Abstract

In this paper we report a combined theoretical and experimental approach to develop enzyme logic gates networks (ELGN). ELGNs were tested in solution with optical analysis of the output signals. Then, the enzymes were immobilized on a buckypaper electrode for electrochemical transduction of the output signals. Finally, the switchable electrodes were integrated in a biofuel cell as a self-powered device for the detection of sucrose in adulterated wine samples.

Original language	English
Title of host publication	FLEPS 2022 - IEEE International Conference on Flexible and Printable Sensors and Systems
Subtitle of host publication	Conference proceedings
Publisher	the Institute of Electrical and Electronics Engineers, Inc.
ISBN (Electronic)	9781665442732
DOIs	https://doi.org/10.1109/FLEPS53764.2022.9781600
Publication status	Published - 2022
MoE publication type	A4 Article in a conference publication
Event	2022 IEEE International Conference on Flexible and Printable Sensors and Systems, FLEPS 2022 - Vienna, Austria Duration: 10 Jul 2022 → 13 Jul 2022

Conference

Conference	2022 IEEE International Conference on Flexible and Printable Sensors and Systems, FLEPS 2022
Country/Territory	Austria
City	Vienna
Period	10/07/22 → 13/07/22

Keywords

biological cascade
Boolean logic gates
enzymes
modified electrodes
ON/OFF sensing

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10.1109/FLEPS53764.2022.9781600Licence: All rights reserved

Cite this

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title = "Enzymatic Boolean Logic Gates toward ON/OFF Sensing",

abstract = "In this paper we report a combined theoretical and experimental approach to develop enzyme logic gates networks (ELGN). ELGNs were tested in solution with optical analysis of the output signals. Then, the enzymes were immobilized on a buckypaper electrode for electrochemical transduction of the output signals. Finally, the switchable electrodes were integrated in a biofuel cell as a self-powered device for the detection of sucrose in adulterated wine samples.",

keywords = "biological cascade, Boolean logic gates, enzymes, modified electrodes, ON/OFF sensing",

author = "Paolo Bollella and Eleonora Macchia and Luisa Torsi",

note = "BOF 15/1 EK Funding Information: The following funding agencies are acknowledged: Academy of Finland projects #316881, #316883 {\textquoteleft}{\textquoteleft}Spatiotemporal control of Cell Functions{\textquoteright}{\textquoteright}, #332106 “ProSiT—Protein Detection at the Single-Molecule Limit with a Self-powered Organic Transistor for HIV early diagnosis”; Biosensori analitici usa-e getta a base di transistori organici auto-alimentati per la rivelazione di biomarcatori proteomici alla singola molecola per la diagnostica decentrata dell{\textquoteright}HIV (6CDD3786); Research for Innovation REFIN—Regione Puglia POR PUGLIA FESR-FSE 2014/2020; Dottorati innovativi con caratterizzazione industrial—PON R&I 2014–2020; “Sensore bio-elettronico usa-e-getta per l{\textquoteright}HIV autoalimentato da una cella a combustibile biologica” (BioElSens&Fuel); SiMBiT— Single molecule bio-electronic smart system array for clinical testing (Grant agreement ID: 824946); PMGB— Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l{\textquoteright}oncologia di precisione— ARS01_01195-PON “RICERCA E INNOVAZIONE” 2014–2020; {\AA}bo Akademi University CoE “Bioelectronic activation of cell functions”; and CSGI are acknowledged for partial financial support. Publisher Copyright: {\textcopyright} 2022 IEEE.; 2022 IEEE International Conference on Flexible and Printable Sensors and Systems, FLEPS 2022 ; Conference date: 10-07-2022 Through 13-07-2022",

year = "2022",

doi = "10.1109/FLEPS53764.2022.9781600",

language = "English",

booktitle = "FLEPS 2022 - IEEE International Conference on Flexible and Printable Sensors and Systems",

publisher = "the Institute of Electrical and Electronics Engineers, Inc.",

}

Bollella, P, Macchia, E & Torsi, L 2022, Enzymatic Boolean Logic Gates toward ON/OFF Sensing. in FLEPS 2022 - IEEE International Conference on Flexible and Printable Sensors and Systems: Conference proceedings. the Institute of Electrical and Electronics Engineers, Inc., 2022 IEEE International Conference on Flexible and Printable Sensors and Systems, FLEPS 2022, Vienna, Austria, 10/07/22. https://doi.org/10.1109/FLEPS53764.2022.9781600

Enzymatic Boolean Logic Gates toward ON/OFF Sensing. / Bollella, Paolo; Macchia, Eleonora; Torsi, Luisa.
FLEPS 2022 - IEEE International Conference on Flexible and Printable Sensors and Systems: Conference proceedings. the Institute of Electrical and Electronics Engineers, Inc., 2022.

Research output: Chapter in Book/Conference proceeding › Conference contribution › Scientific › peer-review

TY - GEN

T1 - Enzymatic Boolean Logic Gates toward ON/OFF Sensing

AU - Bollella, Paolo

AU - Macchia, Eleonora

AU - Torsi, Luisa

N1 - BOF 15/1 EK Funding Information: The following funding agencies are acknowledged: Academy of Finland projects #316881, #316883 ‘‘Spatiotemporal control of Cell Functions’’, #332106 “ProSiT—Protein Detection at the Single-Molecule Limit with a Self-powered Organic Transistor for HIV early diagnosis”; Biosensori analitici usa-e getta a base di transistori organici auto-alimentati per la rivelazione di biomarcatori proteomici alla singola molecola per la diagnostica decentrata dell’HIV (6CDD3786); Research for Innovation REFIN—Regione Puglia POR PUGLIA FESR-FSE 2014/2020; Dottorati innovativi con caratterizzazione industrial—PON R&I 2014–2020; “Sensore bio-elettronico usa-e-getta per l’HIV autoalimentato da una cella a combustibile biologica” (BioElSens&Fuel); SiMBiT— Single molecule bio-electronic smart system array for clinical testing (Grant agreement ID: 824946); PMGB— Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l’oncologia di precisione— ARS01_01195-PON “RICERCA E INNOVAZIONE” 2014–2020; Åbo Akademi University CoE “Bioelectronic activation of cell functions”; and CSGI are acknowledged for partial financial support. Publisher Copyright: © 2022 IEEE.

PY - 2022

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N2 - In this paper we report a combined theoretical and experimental approach to develop enzyme logic gates networks (ELGN). ELGNs were tested in solution with optical analysis of the output signals. Then, the enzymes were immobilized on a buckypaper electrode for electrochemical transduction of the output signals. Finally, the switchable electrodes were integrated in a biofuel cell as a self-powered device for the detection of sucrose in adulterated wine samples.

AB - In this paper we report a combined theoretical and experimental approach to develop enzyme logic gates networks (ELGN). ELGNs were tested in solution with optical analysis of the output signals. Then, the enzymes were immobilized on a buckypaper electrode for electrochemical transduction of the output signals. Finally, the switchable electrodes were integrated in a biofuel cell as a self-powered device for the detection of sucrose in adulterated wine samples.

KW - biological cascade

KW - Boolean logic gates

KW - enzymes

KW - modified electrodes

KW - ON/OFF sensing

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U2 - 10.1109/FLEPS53764.2022.9781600

DO - 10.1109/FLEPS53764.2022.9781600

M3 - Conference contribution

AN - SCOPUS:85137175348

BT - FLEPS 2022 - IEEE International Conference on Flexible and Printable Sensors and Systems

PB - the Institute of Electrical and Electronics Engineers, Inc.

T2 - 2022 IEEE International Conference on Flexible and Printable Sensors and Systems, FLEPS 2022

Y2 - 10 July 2022 through 13 July 2022

ER -

Enzymatic Boolean Logic Gates toward ON/OFF Sensing

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