Sammanfattning
Highly sensitive microRNAs (miRNA) imaging is of great value in the early diagnosis of tumors. Here, we present a novel aggregation-induced emission luminogens (AIEgens) integrated structural color barcode for binary screening of miRNAs. The barcode particles consist of periodically ordered silica nanoparticles encoded cores, and tetraphenylethylene carboxylate derivatives TPE-(COOH)4 integrated inverse opal polyacrylamide (PAAm) hydrogel shells. As the miRNA probes are decorated in the adjacent TPE-(COOH)4 molecules, the skeletal networks of the hydrogel as well as the TPE-(COOH)4 molecules would be closer with the presence of target miRNA due to the formation of a double helix. This phenomenon could not only result in fluorescence change of the TPE-(COOH)4 molecules, but also contribute to structural color variation of shell hydrogel. These two optical signals could be employed as binary channels for more accurate detection of target miRNAs. During this process, the solid cores of the barcodes could keep stable structural colors, thus providing foundation codes for multiplexed detection of miRNAs. Based on these features, we have demonstrated that the AIEgens-integrated structural color barcodes could realize the multiplexed screening of miRNAs in a microfluidics device for in vitro diagnosis, which implies the potential prospects in biomedical fields.
Originalspråk | Engelska |
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Artikelnummer | 144800 |
Antal sidor | 7 |
Tidskrift | Chemical Engineering Journal |
Volym | 471 |
DOI | |
Status | Publicerad - 1 sep. 2023 |
MoE-publikationstyp | A1 Tidskriftsartikel-refererad |
Finansiering
This work was supported by the National Key Research and Development Program of China (2022YFA1105300), the National Natural Science Foundation of China (T2225003, 82102181, 52073060), the Key Research and Development Project of Jiangsu Province (BE20210009), the project funded by China Postdoctoral Science Foundation (2020M681556, 2021T140318) , the Postdoctoral Research Program of Jiangsu Province (2021K123B), the Jiangsu Provincial Double-Innovation Doctor Program (JSSCBS20211486), and the Nanjing Distinguished Young Scholars Foundation (JQX22002).
Finansiärer | Finansiärsnummer |
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Jiangsu Provincial Double-Innovation Doctor Program | JSSCBS20211486 |
Nanjing Distinguished Young Scholars Foundation | JQX22002 |
Postdoctoral Research Program of Jiangsu Province | 2021K123B |
National Natural Science Foundation of China | 82102181, 52073060, T2225003 |
China Postdoctoral Science Foundation | 2021T140318, 2020M681556 |
National Key Research and Development Program of China | 2022YFA1105300 |
Jiangsu Provincial Key Research and Development Program | BE20210009 |