Photocross-Linkable and Shape-Memory Biomaterial Hydrogel Based on Methacrylated Cellulose Nanofibres

Yury Brusentsev, Peiru Yang, Alistair W. T. King, Fang Cheng, Maria F. Cortes Ruiz, John E. Eriksson, Ilkka Kilpeläinen, Stefan Willför, Chunlin Xu, Lars Wågberg, Xiaoju Wang

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

6 Sitaatiot (Scopus)
27 Lataukset (Pure)


In the context of three-dimensional (3D) cell culture and tissue engineering, 3D printing is a powerful tool for customizing in vitro 3D cell culture models that are critical for understanding the cell–matrix and cell–cell interactions. Cellulose nanofibril (CNF) hydrogels are emerging in constructing scaffolds able to imitate tissue in a microenvironment. A direct modification of the methacryloyl (MA) group onto CNF is an appealing approach to synthesize photocross-linkable building blocks in formulating CNF-based bioinks for light-assisted 3D printing; however, it faces the challenge of the low efficiency of heterogenous surface modification. Here, a multistep approach yields CNF methacrylate (CNF-MA) with a decent degree of substitution while maintaining a highly dispersible CNF hydrogel, and CNF-MA is further formulated and copolymerized with monomeric acrylamide (AA) to form a super transparent hydrogel with tuneable mechanical strength (compression modulus, approximately 5–15 kPa). The resulting photocurable hydrogel shows good printability in direct ink writing and good cytocompatibility with HeLa and human dermal fibroblast cell lines. Moreover, the hydrogel reswells in water and expands to all directions to restore its original dimension after being air-dried, with further enhanced mechanical properties, for example, Young’s modulus of a 1.1% CNF-MA/1% PAA hydrogel after reswelling in water increases to 10.3 kPa from 5.5 kPa.
DOI - pysyväislinkit
TilaJulkaistu - 14 elok. 2023
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu


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