TY - JOUR
T1 - Mucoadhesive chitosan- and cellulose derivative-based nanofiber-on-foam-on-film system for non-invasive peptide delivery
AU - Stie, Mai Bay
AU - Öblom, Heidi
AU - Hansen, Anders Christian Nørgaard
AU - Jacobsen, Jette
AU - Chronakis, Ioannis S.
AU - Rantanen, Jukka
AU - Nielsen, Hanne Mørck
AU - Genina, Natalja
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Oromucosal administration is an attractive non-invasive route. However, drug absorption is challenged by salivary flow and the mucosa being a significant permeability barrier. The aim of this study was to design and investigate a multi-layered nanofiber-on-foam-on-film (NFF) drug delivery system with unique properties and based on polysaccharides combined as i) mucoadhesive chitosan-based nanofibers, ii) a peptide loaded hydroxypropyl methylcellulose foam, and iii) a saliva-repelling backing film based on ethylcellulose. NFF displays optimal mechanical properties shown by dynamic mechanical analysis, and biocompatibility demonstrated after exposure to a TR146 cell monolayer. Chitosan-based nanofibers provided the NFF with improved mucoadhesion compared to that of the foam alone. After 1 h, >80 % of the peptide desmopressin was released from the NFF. Ex vivo permeation studies across porcine buccal mucosa indicated that NFF improved the permeation of desmopressin compared to a commercial freeze-dried tablet. The findings demonstrate the potential of the NFF as a biocompatible drug delivery system.
AB - Oromucosal administration is an attractive non-invasive route. However, drug absorption is challenged by salivary flow and the mucosa being a significant permeability barrier. The aim of this study was to design and investigate a multi-layered nanofiber-on-foam-on-film (NFF) drug delivery system with unique properties and based on polysaccharides combined as i) mucoadhesive chitosan-based nanofibers, ii) a peptide loaded hydroxypropyl methylcellulose foam, and iii) a saliva-repelling backing film based on ethylcellulose. NFF displays optimal mechanical properties shown by dynamic mechanical analysis, and biocompatibility demonstrated after exposure to a TR146 cell monolayer. Chitosan-based nanofibers provided the NFF with improved mucoadhesion compared to that of the foam alone. After 1 h, >80 % of the peptide desmopressin was released from the NFF. Ex vivo permeation studies across porcine buccal mucosa indicated that NFF improved the permeation of desmopressin compared to a commercial freeze-dried tablet. The findings demonstrate the potential of the NFF as a biocompatible drug delivery system.
KW - Biopharmaceuticals
KW - Chitosan
KW - Hydroxypropyl methylcellulose
KW - Mucoadhesion
KW - Oromucosal drug delivery
KW - Peptide
UR - http://www.scopus.com/inward/record.url?scp=85144048666&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2022.120429
DO - 10.1016/j.carbpol.2022.120429
M3 - Article
C2 - 36657829
AN - SCOPUS:85144048666
SN - 0144-8617
VL - 303
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 120429
ER -