Projekteja vuodessa
Abstrakti
Recent experimental evidence indicates a role for the intermediate filament vimentin in regulating cellular mechanical homeostasis, but its precise contribution remains to be discovered. Mechanical homeostasis requires a balanced bi-directional interplay between the cell's microenvironment and the cellular morphological and mechanical state-this balance being regulated via processes of mechanotransduction and mechanoresponse, commonly referred to as mechanoreciprocity. Here, we systematically analyze vimentin-expressing and vimentin-depleted cells in a swatch of in vitro cellular microenvironments varying in stiffness and/or ECM density. We find that vimentin-expressing cells maintain mechanical homeostasis by adapting cellular morphology and mechanics to micromechanical changes in the microenvironment. However, vimentin-depleted cells lose this mechanoresponse ability on short timescales, only to reacquire it on longer time scales. Indeed, we find that the morphology and mechanics of vimentin-depleted cell in stiffened microenvironmental conditions can get restored to the homeostatic levels of vimentin-expressing cells. Additionally, we observed vimentin-depleted cells increasing collagen matrix synthesis and its crosslinking, a phenomenon which is known to increase matrix stiffness, and which we now hypothesize to be a cellular compensation mechanism for the loss of vimentin. Taken together, our findings provide further insight in the regulating role of intermediate filament vimentin in mediating mechanoreciprocity and mechanical homeostasis.
Alkuperäiskieli | Englanti |
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Artikkeli | 18374 |
Sivumäärä | 12 |
Julkaisu | Scientific Reports |
Vuosikerta | 13 |
DOI - pysyväislinkit | |
Tila | Julkaistu - 26 lokak. 2023 |
OKM-julkaisutyyppi | A1 Julkaistu artikkeli, soviteltu |
Sormenjälki
Sukella tutkimusaiheisiin 'Environmental stiffness restores mechanical homeostasis in vimentin-depleted cells'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.Projektit
- 4 Päättynyt
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SignalSheets: Multilayer mechanosignalling in vascular homeostasis
Sahlgren, C. (Vastuullinen tutkija)
01/09/20 → 31/08/24
Projekti: Research Council of Finland/Other Research Councils
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SPACE: Spatiotemporal Control of Cell Functions
Österbacka, R. (Vastuullinen tutkija), Sahlgren, C. (CoPI), Torsi, L. (CoPI) & Ghafarihashjin, A. (CoI)
01/09/18 → 31/08/22
Projekti: Research Council of Finland/Other Research Councils
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ForceMorph: The integration of cell signalling and mechanical forces in vascular morphology
Sahlgren, C. (Vastuullinen tutkija)
01/03/18 → 31/08/23
Projekti: EU