TY - JOUR
T1 - Engagement of vimentin intermediate filaments in hypotonic stress
AU - Li, Jian
AU - Gao, Wei
AU - Zhang, Yue
AU - Cheng, Fang
AU - Eriksson, John E
AU - Etienne-Manneville, Sandrine
AU - Jiu, Yaming
N1 - © 2019 Wiley Periodicals, Inc.
PY - 2019/8
Y1 - 2019/8
N2 - Intermediate filaments (IFs) play a key role in the control of cell structure and morphology, cell mechano-responses, migration, proliferation, and apoptosis. However, the mechanisms regulating IFs organization in motile adhesive cells under certain physical/pathological conditions remain to be fully understood. In this study, we found hypo-osmotic-induced stress results in a dramatic but reversible rearrangement of the IF network. Vimentin and nestin IFs are partially depolymerized as they are redistributed throughout the cell cytoplasm after hypo-osmotic shock. This spreading of the IFs requires an intact microtubule network and the motor protein associated transportation. Both nocodazole treatment and depletion of kinesin-1 (KIF5B) block the hypo-osmotic shock-induced rearrangement of IFs showing that the dynamic behavior of IFs largely depends on microtubules and kinesin-dependent transport. Moreover, we show that cell survival rates are dramatically decreased in response to hypo-osmotic shock, which was more severe by vimentin IFs depletion, indicating its contribution to osmotic endurance. Collectively, these results reveal a critical role of vimentin IFs under hypotonic stress and provide evidence that IFs are important for the defense mechanisms during the osmotic challenge.
AB - Intermediate filaments (IFs) play a key role in the control of cell structure and morphology, cell mechano-responses, migration, proliferation, and apoptosis. However, the mechanisms regulating IFs organization in motile adhesive cells under certain physical/pathological conditions remain to be fully understood. In this study, we found hypo-osmotic-induced stress results in a dramatic but reversible rearrangement of the IF network. Vimentin and nestin IFs are partially depolymerized as they are redistributed throughout the cell cytoplasm after hypo-osmotic shock. This spreading of the IFs requires an intact microtubule network and the motor protein associated transportation. Both nocodazole treatment and depletion of kinesin-1 (KIF5B) block the hypo-osmotic shock-induced rearrangement of IFs showing that the dynamic behavior of IFs largely depends on microtubules and kinesin-dependent transport. Moreover, we show that cell survival rates are dramatically decreased in response to hypo-osmotic shock, which was more severe by vimentin IFs depletion, indicating its contribution to osmotic endurance. Collectively, these results reveal a critical role of vimentin IFs under hypotonic stress and provide evidence that IFs are important for the defense mechanisms during the osmotic challenge.
KW - Cell Line, Tumor
KW - Fluorescent Antibody Technique
KW - HeLa Cells
KW - Humans
KW - Intermediate Filaments/metabolism
KW - Microtubules/drug effects
KW - Nocodazole/pharmacology
KW - Osmotic Pressure/drug effects
KW - Vimentin/metabolism
U2 - 10.1002/jcb.28591
DO - 10.1002/jcb.28591
M3 - Article
C2 - 30887571
SN - 0730-2312
VL - 120
SP - 13168
EP - 13176
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 8
ER -