Printed biotin-functionalised polythiophene films as biorecognition layers in the development of paper-based biosensors

Petri Ihalainen; Markus Pesonen; Pernilla Sund; Tapani Viitala; Anni Määttänen; Jawad Sarfraz; Carl Erik Wilén; Ronald Österbacka; Jouko Peltonen

doi:10.1016/j.apsusc.2015.12.187

Printed biotin-functionalised polythiophene films as biorecognition layers in the development of paper-based biosensors

Petri Ihalainen^*, Markus Pesonen, Pernilla Sund, Tapani Viitala, Anni Määttänen, Jawad Sarfraz, Carl Erik Wilén, Ronald Österbacka, Jouko Peltonen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

7 Citations (Scopus)

Abstract

The integration of flexible electronic sensors in clinical diagnostics is visioned to significantly reduce the cost of many diagnostic tests and ultimately make healthcare more accessible. This study concentrates on the characterisation of inkjet-printed bio-functionalised polythiophene films on paper-based ultrathin gold film (UTGF) electrodes and their possible application as biorecognition layers. Physicochemical surface properties (topography, chemistry, and wetting) and electrochemical characteristics of water-soluble regioirregular tetraethylene-glycol polythiophene (TEGPT) and biotin-functionalised TEGPT (b-TEGPT) films were examined and compared. In addition, their specificity towards streptavidin protein was tested. The results show that stable supramolecular biorecognition layers of insulating b-TEGPT and streptavidin were successfully fabricated on a paper-based UTGF by inkjet-printing. Good adhesion of thiophene to UTGF can be attributed to covalent linkage between sulphur and gold, whereas the stability of the streptavidin layer is due to the high affinity between biotin and streptavidin. The device introduced can be utilised in the development of biosensors for clinically relevant analytes e.g. for detecting complementary DNA oligomers or antibody-antigen complexes.

Original language	English
Pages (from-to)	477-483
Number of pages	7
Journal	Applied Surface Science
Volume	364
DOIs	https://doi.org/10.1016/j.apsusc.2015.12.187
Publication status	Published - 28 Feb 2016
MoE publication type	A1 Journal article-refereed

Keywords

Bio-recognition layer
Polythiophene
Inkjet printing
Streptavidin
Ultrathin gold film
Paper substrate

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10.1016/j.apsusc.2015.12.187

http://www.sciencedirect.com/science/article/pii/S016943321503192X

Cite this

@article{418ee3c588b7405792144403f43ddff6,

title = "Printed biotin-functionalised polythiophene films as biorecognition layers in the development of paper-based biosensors",

abstract = "The integration of flexible electronic sensors in clinical diagnostics is visioned to significantly reduce the cost of many diagnostic tests and ultimately make healthcare more accessible. This study concentrates on the characterisation of inkjet-printed bio-functionalised polythiophene films on paper-based ultrathin gold film (UTGF) electrodes and their possible application as biorecognition layers. Physicochemical surface properties (topography, chemistry, and wetting) and electrochemical characteristics of water-soluble regioirregular tetraethylene-glycol polythiophene (TEGPT) and biotin-functionalised TEGPT (b-TEGPT) films were examined and compared. In addition, their specificity towards streptavidin protein was tested. The results show that stable supramolecular biorecognition layers of insulating b-TEGPT and streptavidin were successfully fabricated on a paper-based UTGF by inkjet-printing. Good adhesion of thiophene to UTGF can be attributed to covalent linkage between sulphur and gold, whereas the stability of the streptavidin layer is due to the high affinity between biotin and streptavidin. The device introduced can be utilised in the development of biosensors for clinically relevant analytes e.g. for detecting complementary DNA oligomers or antibody-antigen complexes.",

keywords = "Bio-recognition layer, Polythiophene, Inkjet printing, Streptavidin, Ultrathin gold film, Paper substrate",

author = "Petri Ihalainen and Markus Pesonen and Pernilla Sund and Tapani Viitala and Anni M{\"a}{\"a}tt{\"a}nen and Jawad Sarfraz and Wil{\'e}n, {Carl Erik} and Ronald {\"O}sterbacka and Jouko Peltonen",

note = "Funding Information: The SalWe IMO programme (Project number: 648/10 ) funded by the Finnish Funding Agency for Technology and Innovation (TEKES) is acknowledged. TVA acknowledges Academy of Finland for Academy Research Fellow funding (grant no. 137053 ). Publisher Copyright: {\textcopyright} 2015 Elsevier B.V. All rights reserved.",

year = "2016",

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day = "28",

doi = "10.1016/j.apsusc.2015.12.187",

language = "English",

volume = "364",

pages = "477--483",

journal = "Applied Surface Science",

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T1 - Printed biotin-functionalised polythiophene films as biorecognition layers in the development of paper-based biosensors

AU - Ihalainen, Petri

AU - Pesonen, Markus

AU - Sund, Pernilla

AU - Viitala, Tapani

AU - Määttänen, Anni

AU - Sarfraz, Jawad

AU - Wilén, Carl Erik

AU - Österbacka, Ronald

AU - Peltonen, Jouko

N1 - Funding Information: The SalWe IMO programme (Project number: 648/10 ) funded by the Finnish Funding Agency for Technology and Innovation (TEKES) is acknowledged. TVA acknowledges Academy of Finland for Academy Research Fellow funding (grant no. 137053 ). Publisher Copyright: © 2015 Elsevier B.V. All rights reserved.

PY - 2016/2/28

Y1 - 2016/2/28

N2 - The integration of flexible electronic sensors in clinical diagnostics is visioned to significantly reduce the cost of many diagnostic tests and ultimately make healthcare more accessible. This study concentrates on the characterisation of inkjet-printed bio-functionalised polythiophene films on paper-based ultrathin gold film (UTGF) electrodes and their possible application as biorecognition layers. Physicochemical surface properties (topography, chemistry, and wetting) and electrochemical characteristics of water-soluble regioirregular tetraethylene-glycol polythiophene (TEGPT) and biotin-functionalised TEGPT (b-TEGPT) films were examined and compared. In addition, their specificity towards streptavidin protein was tested. The results show that stable supramolecular biorecognition layers of insulating b-TEGPT and streptavidin were successfully fabricated on a paper-based UTGF by inkjet-printing. Good adhesion of thiophene to UTGF can be attributed to covalent linkage between sulphur and gold, whereas the stability of the streptavidin layer is due to the high affinity between biotin and streptavidin. The device introduced can be utilised in the development of biosensors for clinically relevant analytes e.g. for detecting complementary DNA oligomers or antibody-antigen complexes.

AB - The integration of flexible electronic sensors in clinical diagnostics is visioned to significantly reduce the cost of many diagnostic tests and ultimately make healthcare more accessible. This study concentrates on the characterisation of inkjet-printed bio-functionalised polythiophene films on paper-based ultrathin gold film (UTGF) electrodes and their possible application as biorecognition layers. Physicochemical surface properties (topography, chemistry, and wetting) and electrochemical characteristics of water-soluble regioirregular tetraethylene-glycol polythiophene (TEGPT) and biotin-functionalised TEGPT (b-TEGPT) films were examined and compared. In addition, their specificity towards streptavidin protein was tested. The results show that stable supramolecular biorecognition layers of insulating b-TEGPT and streptavidin were successfully fabricated on a paper-based UTGF by inkjet-printing. Good adhesion of thiophene to UTGF can be attributed to covalent linkage between sulphur and gold, whereas the stability of the streptavidin layer is due to the high affinity between biotin and streptavidin. The device introduced can be utilised in the development of biosensors for clinically relevant analytes e.g. for detecting complementary DNA oligomers or antibody-antigen complexes.

KW - Bio-recognition layer

KW - Polythiophene

KW - Inkjet printing

KW - Streptavidin

KW - Ultrathin gold film

KW - Paper substrate

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DO - 10.1016/j.apsusc.2015.12.187

M3 - Article

SN - 0169-4332

VL - 364

SP - 477

EP - 483

JO - Applied Surface Science

JF - Applied Surface Science

ER -

Printed biotin-functionalised polythiophene films as biorecognition layers in the development of paper-based biosensors

Abstract

Keywords

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