TY - JOUR
T1 - Lab-made flexible third-generation fructose biosensors based on 0D-nanostructured transducers
AU - Silveri, Filippo
AU - Paolini, Davide
AU - Della Pelle, Flavio
AU - Bollella, Paolo
AU - Scroccarello, Annalisa
AU - Suzuki, Yohei
AU - Fukawa, Eole
AU - Sowa, Keisei
AU - Di Franco, Cinzia
AU - Torsi, Luisa
AU - Compagnone, Dario
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/10/1
Y1 - 2023/10/1
N2 - Herein, we report a scalable benchtop electrode fabrication method to produce highly sensitive and flexible third-generation fructose dehydrogenase amperometric biosensors based on water-dispersed 0D-nanomaterials. The electrochemical platform was fabricated via Stencil-Printing (StPE) and insulated via xurography. Carbon black (CB) and mesoporous carbon (MS) were employed as 0D-nanomaterials promoting an efficient direct electron transfer (DET) between fructose dehydrogenase (FDH) and the transducer. Both nanomaterials were prepared in water-phase via a sonochemical approach. The nano-StPE exhibited enhanced electrocatalytic currents compared to conventional commercial electrodes. The enzymatic sensors were exploited for the determination of D-fructose in model solutions and various food and biological samples. StPE-CB and StPE-MS integrated biosensors showed appreciable sensitivity (∼150 μA cm−2 mM−1) with μmolar limit of detection (0.35 and 0.16 μM, respectively) and extended linear range (2–500 and 1–250 μM, respectively); the selectivity of the biosensors, ensured by the low working overpotential (+0.15 V), has been also demonstrated. Good accuracy (recoveries between 95 and 116%) and reproducibility (RSD ≤8.6%) were achieved for food and urine samples. The proposed approach because of manufacturing versatility and the electro-catalytic features of the water-nanostructured 0D-NMs opens new paths for affordable and customizable FDH-based bioelectronics.
AB - Herein, we report a scalable benchtop electrode fabrication method to produce highly sensitive and flexible third-generation fructose dehydrogenase amperometric biosensors based on water-dispersed 0D-nanomaterials. The electrochemical platform was fabricated via Stencil-Printing (StPE) and insulated via xurography. Carbon black (CB) and mesoporous carbon (MS) were employed as 0D-nanomaterials promoting an efficient direct electron transfer (DET) between fructose dehydrogenase (FDH) and the transducer. Both nanomaterials were prepared in water-phase via a sonochemical approach. The nano-StPE exhibited enhanced electrocatalytic currents compared to conventional commercial electrodes. The enzymatic sensors were exploited for the determination of D-fructose in model solutions and various food and biological samples. StPE-CB and StPE-MS integrated biosensors showed appreciable sensitivity (∼150 μA cm−2 mM−1) with μmolar limit of detection (0.35 and 0.16 μM, respectively) and extended linear range (2–500 and 1–250 μM, respectively); the selectivity of the biosensors, ensured by the low working overpotential (+0.15 V), has been also demonstrated. Good accuracy (recoveries between 95 and 116%) and reproducibility (RSD ≤8.6%) were achieved for food and urine samples. The proposed approach because of manufacturing versatility and the electro-catalytic features of the water-nanostructured 0D-NMs opens new paths for affordable and customizable FDH-based bioelectronics.
KW - Direct electron transfer
KW - Enzyme-based sensors
KW - Flexible amperometric biosensors
KW - Fructose dehydrogenase
KW - Nanomaterials
KW - Point-of-care/needs devices
UR - http://www.scopus.com/inward/record.url?scp=85162081645&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2023.115450
DO - 10.1016/j.bios.2023.115450
M3 - Article
C2 - 37343312
AN - SCOPUS:85162081645
SN - 0956-5663
VL - 237
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 115450
ER -