Lab-made flexible third-generation fructose biosensors based on 0D-nanostructured transducers

Filippo Silveri, Davide Paolini, Flavio Della Pelle*, Paolo Bollella, Annalisa Scroccarello, Yohei Suzuki, Eole Fukawa, Keisei Sowa, Cinzia Di Franco, Luisa Torsi, Dario Compagnone*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)
10 Downloads (Pure)


Herein, we report a scalable benchtop electrode fabrication method to produce highly sensitive and flexible third-generation fructose dehydrogenase amperometric biosensors based on water-dispersed 0D-nanomaterials. The electrochemical platform was fabricated via Stencil-Printing (StPE) and insulated via xurography. Carbon black (CB) and mesoporous carbon (MS) were employed as 0D-nanomaterials promoting an efficient direct electron transfer (DET) between fructose dehydrogenase (FDH) and the transducer. Both nanomaterials were prepared in water-phase via a sonochemical approach. The nano-StPE exhibited enhanced electrocatalytic currents compared to conventional commercial electrodes. The enzymatic sensors were exploited for the determination of D-fructose in model solutions and various food and biological samples. StPE-CB and StPE-MS integrated biosensors showed appreciable sensitivity (∼150 μA cm−2 mM−1) with μmolar limit of detection (0.35 and 0.16 μM, respectively) and extended linear range (2–500 and 1–250 μM, respectively); the selectivity of the biosensors, ensured by the low working overpotential (+0.15 V), has been also demonstrated. Good accuracy (recoveries between 95 and 116%) and reproducibility (RSD ≤8.6%) were achieved for food and urine samples. The proposed approach because of manufacturing versatility and the electro-catalytic features of the water-nanostructured 0D-NMs opens new paths for affordable and customizable FDH-based bioelectronics.

Original languageEnglish
Article number115450
JournalBiosensors and Bioelectronics
Publication statusPublished - 1 Oct 2023
MoE publication typeA1 Journal article-refereed


  • Direct electron transfer
  • Enzyme-based sensors
  • Flexible amperometric biosensors
  • Fructose dehydrogenase
  • Nanomaterials
  • Point-of-care/needs devices


Dive into the research topics of 'Lab-made flexible third-generation fructose biosensors based on 0D-nanostructured transducers'. Together they form a unique fingerprint.

Cite this