Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid

Yatsunami, Fujiki, Suganuma, Yoshizawa, John Eriksson, Olson, Goldman

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    Sammanfattning

    Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)1165–1170
    TidskriftBiochemical and Biophysical Research Communications
    Volym177
    Utgåva3
    DOI
    StatusPublicerad - 1991
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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