Transcriptional response to stress in the dynamic chromatin environment of cycling and mitotic cells

Anniina Vihervaara, C Sergelius, J Vasara, Blom MAH, Alexandra Elsing, Pia Roos-Mattjus, Lea Sistonen

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    87 Citeringar (Scopus)

    Sammanfattning

    Heat shock factors (HSFs) are the master regulators of transcription under protein-damaging conditions, acting in an environment where the overall transcription is silenced. We determined the genomewide transcriptional program that is rapidly provoked by HSF1 and HSF2 under acute stress in human cells. Our results revealed the molecular mechanisms that maintain cellular homeostasis, including HSF1-driven induction of polyubiquitin genes, as well as HSF1- and HSF2-mediated expression patterns of cochaperones, transcriptional regulators, and signaling molecules. We characterized the genomewide transcriptional response to stress also in mitotic cells where the chromatin is tightly compacted. We found a radically limited binding and transactivating capacity of HSF1, leaving mitotic cells highly susceptible to proteotoxicity. In contrast, HSF2 occupied hundreds of loci in the mitotic cells and localized to the condensed chromatin also in meiosis. These results highlight the importance of the cell cycle phase in transcriptional responses and identify the specific mechanisms for HSF1 and HSF2 in transcriptional orchestration. Moreover, we propose that HSF2 is an epigenetic regulator directing transcription throughout cell cycle progression.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)E3388–E3397
    Antal sidor10
    TidskriftProceedings of the National Academy of Sciences
    Volym110
    Utgåva36
    DOI
    StatusPublicerad - 2013
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Nyckelord

    • ChIP-seq
    • ENCODE
    • human genome
    • proteostasis

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