Ru modified MCM-41 mesoporous material, Y and Beta zeolites were synthesized, characterized and investigated in ring opening of decalin. Ru-MCM-41 catalysts were prepared using ion-exchange, impregnation and in situ synthesis methods. Ru-MCM-41, Ru-Y and Ru-Beta zeolite catalysts were characterized using XRD, SEM, EDXA, FTIR, XRF and nitrogen adsorption. Ru modification of MCM-41, Y and Beta zeolites did not influence the parent phase purity of materials. Microporous H-Beta and H-Y catalysts showed larger number of Brønsted acid sites than H-MCM-41 mesoporous material. Method of Ru introduction in MCM-41 was observed to influence conversion of ring opening of decalin and selectivity to ring opening products. Ru-MCM-41-IE catalyst prepared by ion-exchange method exhibited higher conversion of decalin than Ru-MCM-41-IMP and Ru-MCM-41-IS catalysts prepared by impregnation and in situ synthesis methods. Ru-MCM-41-IS catalyst prepared by in situ method showed higher selectivity to ring opening products than Ru-MCM-41-IE and Ru-MCM-41-IMP catalysts.