Studies Related to Norway Spruce Galactoglucomannans: Chemical Synthesis, Conformation Analysis, NMR Spectroscopic Characterization, and Molecular Recognition of Model Compounds

Filip S. Ekholm, Ana Ardá, Patrik Christoffer Eklund, Sabine André, Hans-Joachim Gabius, Jesús Jiménez-Barbero, Reko Leino

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    24 Citeringar (Scopus)

    Sammanfattning

    Galactoglucomannan (GGM) is a polysaccharide mainly consisting of mannose, glucose, and galactose. GGM is the most abundant hemicellulose in the Norway spruce (Picea abies), but is also found in the cell wall of flax seeds, tobacco plants, and kiwifruit. Although several applications for GGM polysaccharides have been developed in pulp and paper manufacturing and the food and medical industries, attempts to synthesize and study distinct fragments of this polysaccharide have not been reported previously. Herein, the synthesis of one of the core trisaccharide units of GGM together with a less-abundant tetrasaccharide fragment is described. In addition, detailed NMR spectroscopic characterization of the model compounds, comparison of the spectral data with natural GGM, investigation of the acetyl-group migration phenomena that takes place in the polysaccharide by using small model compounds, and a binding study between the tetrasaccharide model fragment and a galactose-binding protein (the toxin viscumin) are reported.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)14392–14405
    Antal sidor14
    TidskriftChemistry - A European Journal
    Volym18
    Utgåva45
    DOI
    StatusPublicerad - 2012
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Nyckelord

    • conformation analysis
    • galactoglucomannan
    • molecular recognition
    • NMR spectroscopy
    • oligosaccharide synthesis

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