Structural Catalytic Core in Subtilisin-like Proteins and Its Comparison to Trypsin-like Serine Proteases and Alpha/Beta-Hydrolases

Alexandre Denesyuk; Konstantin Denessiouk; Mark Johnson; Vladimir Uversky

doi:10.3390/ijms252211858

Structural Catalytic Core in Subtilisin-like Proteins and Its Comparison to Trypsin-like Serine Proteases and Alpha/Beta-Hydrolases

Alexandre Denesyuk^*
, Konstantin Denessiouk
, Mark Johnson
, Vladimir Uversky^*

^*Korresponderande författare för detta arbete

Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review

10 Citeringar (Scopus)

178 Nedladdningar (Pure)

Sammanfattning

Subtilisin-like proteins are serine proteases that use two types of catalytic triads: Ser-His-Asp and Ser-Glu-Asp. Here, we investigate the two known families of subtilisin-like proteins, the subtilases (Ser-His-Asp triad) and the serine-carboxyl proteinases (Ser-Glu-Asp triad), and describe the local structural arrangements (cores) that govern the catalytic residues in these proteins. We show the separation of the cores into conserved structural zones, which can be repeatedly found in different structures, and compare the structural cores in subtilisin-like proteins with those in trypsin-like serine proteases and alpha/beta-hydrolases.

Originalspråk	Engelska
Artikelnummer	11858
Sidor (från-till)	11858
Tidskrift	International Journal of Molecular Sciences
Volym	25
Nummer	22
DOI	https://doi.org/10.3390/ijms252211858
Status	Publicerad - 5 nov. 2024
MoE-publikationstyp	A1 Tidskriftsartikel-refererad

Finansiering

We thank the Biocenter Finland Bioinformatics Network (Jukka Lehtonen) and CSC IT Center for Science for computational support for the project. The Structural Bioinformatics Laboratory is part of the Solution for Health strategic area of \u00C5bo Akademi University and within the InFLAMES Flagship program for inflammation and infection, \u00C5bo Akademi University and the University of Turku, funded by the Academy of Finland.

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10.3390/ijms252211858

ijms-25-11858Slutlig publicerad version, 3,2 MBLicens: CC BY

https://urn.fi/URN:NBN:fi-fe20241213102315

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Structural Catalytic Core in Subtilisin-like Proteins and Its Comparison to Trypsin-like Serine Proteases and Alpha/Beta-Hydrolases. / Denesyuk, Alexandre ; Denessiouk, Konstantin ; Johnson, Mark et al.
I: International Journal of Molecular Sciences, Vol. 25, Nr. 22, 11858, 05.11.2024, s. 11858.

Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review

TY - JOUR

T1 - Structural Catalytic Core in Subtilisin-like Proteins and Its Comparison to Trypsin-like Serine Proteases and Alpha/Beta-Hydrolases

AU - Denesyuk, Alexandre

AU - Denessiouk, Konstantin

AU - Johnson, Mark

AU - Uversky, Vladimir

PY - 2024/11/5

Y1 - 2024/11/5

N2 - Subtilisin-like proteins are serine proteases that use two types of catalytic triads: Ser-His-Asp and Ser-Glu-Asp. Here, we investigate the two known families of subtilisin-like proteins, the subtilases (Ser-His-Asp triad) and the serine-carboxyl proteinases (Ser-Glu-Asp triad), and describe the local structural arrangements (cores) that govern the catalytic residues in these proteins. We show the separation of the cores into conserved structural zones, which can be repeatedly found in different structures, and compare the structural cores in subtilisin-like proteins with those in trypsin-like serine proteases and alpha/beta-hydrolases.

AB - Subtilisin-like proteins are serine proteases that use two types of catalytic triads: Ser-His-Asp and Ser-Glu-Asp. Here, we investigate the two known families of subtilisin-like proteins, the subtilases (Ser-His-Asp triad) and the serine-carboxyl proteinases (Ser-Glu-Asp triad), and describe the local structural arrangements (cores) that govern the catalytic residues in these proteins. We show the separation of the cores into conserved structural zones, which can be repeatedly found in different structures, and compare the structural cores in subtilisin-like proteins with those in trypsin-like serine proteases and alpha/beta-hydrolases.

KW - alpha/beta-hydrolases

KW - catalytic pentad

KW - serine proteases

KW - subtilisin-like

U2 - 10.3390/ijms252211858

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ER -

Structural Catalytic Core in Subtilisin-like Proteins and Its Comparison to Trypsin-like Serine Proteases and Alpha/Beta-Hydrolases

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