Solid-Supported Porphyrins Useful for the Synthesis of Conjugates with Oligomeric Biomolecules

Satish Jadhav, Cheng-Bin Yim, Johan Rajander, Tove J. Grönroos, Olof Solin, Pasi Virta

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

6 Citeringar (Scopus)

Sammanfattning

meso-Tris(pyridin-4-yl)(4-carboxyphenyl)porphyrin and 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (Photochlor, HPPH) were amide-coupled to 1R,2S,3R,4R-2,3-dihydroxy-4-(hydromethyl)-1-aminocyclopentane and immobilized via an ester linkage to long chain alkyl amine-derivatized controlled pore glass (LCAA-CPG). The applicability of these supports (5 and 6) for the synthesis of porphyrin conjugates with oligomeric biomolecules was demonstrated using an automated phosphoramidite coupling chemistry. Cleavage from the support with concentrated ammonia gave the products, viz., porphyrin conjugates of oligonucleotides (79) and dendritic glycoclusters (1013) and a cyclooctyne derivative (14) in 23–58% yield. In addition, the synthesized cyclooctyne derivative of meso-tris(pyridin-4-yl)(4-carboxyphenyl)porphyrin (14) was conjugated with an azidopropyl-modified hyaluronic acid (19). The hyaluronic acid–porphyrin conjugate (15) was radiolabeled with 64Cu and its (15[64Cu]) receptor binding affinity to CD44-expressing tumor cells was evaluated.
OriginalspråkOdefinierat/okänt
Sidor (från-till)1023–1029
TidskriftBioconjugate Chemistry
Volym27
Utgåva4
DOI
StatusPublicerad - 2016
MoE-publikationstypA1 Tidskriftsartikel-refererad

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