TY - JOUR
T1 - Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies
AU - Özliseli, Ezgi
AU - Ṣen Karaman, Didem
AU - Chakraborti, Soumyananda
AU - Slita, Anna
AU - Parikainen, Marjaana
AU - Sahlgren, Cecilia M.
AU - Rosenholm, Jessica M.
N1 - Funding Information:
Sigrid Jusélius Foundation (Finland) , Swedish Cultural Foundation (Finland) , and Doctoral Network for Molecular Biosciences of Åbo Akademi University are gratefully acknowledged providing financial support to E.Ö. D.S.K. thankfully acknowledge the researching funding (#2018-GAP-MÜMF-0008) from Scientific Research Projects Coordination Unit of Izmir Katip Çelebi University (IKCU), additional to the support provided by Biomedical Engineering Central Research Laboratories of Izmir Kâtip Çelebi University . -. C.S. and M.P. are supported by The Academy of Finland (# 307133). K. Albin Johansson Foundation is acknowledged for financial support to M.P. The Academy of Finland (# 309374 ) is also greatly acknowledged for financial support to J . M.R.
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9/5
Y1 - 2020/9/5
N2 - The supplementation of stem cell culture medium with fetal bovine serum (FBS) for maintenance and propagation of cell lines in vitro is associated with immunogenicity and disease transmission by prions, bacteria, and viruses. Therefore, establishing xenogenic-free cell culture media for the benefit of rational testing conditions for stem cells in terms of eliminating the disadvantages is essential. In parallel, a number of investigations have been carried out on nanoparticle (NP) aided stem cell-based therapies. To use NP-integrated stem cell therapy in clinical applications, NP behavior in xenogenic-free stem cell cultures needs to be understood in detail. Mesoporous silica nanoparticles (MSN) are profusely used in biomedical applications and have also shown great potential in stem cell therapies. One of the strategies to make them compatible with stem cell therapy is to alter their surface functionalization. In line with this notion, the main interest of the present investigation was to study the impact of human serum albumin (HSA) association with differently surface-modified MSN, and rationalize the MSN utilization in xenogenic-free stem cell culture by employing C2C12 myogenic progenitor cells as a model system. Our results revealed that HSA coating on differently surface-modified MSN is a promising strategy to improve the colloidal stability of MSN, stem cell viability, and imparts no adverse effects on the differentiation of stem cells.
AB - The supplementation of stem cell culture medium with fetal bovine serum (FBS) for maintenance and propagation of cell lines in vitro is associated with immunogenicity and disease transmission by prions, bacteria, and viruses. Therefore, establishing xenogenic-free cell culture media for the benefit of rational testing conditions for stem cells in terms of eliminating the disadvantages is essential. In parallel, a number of investigations have been carried out on nanoparticle (NP) aided stem cell-based therapies. To use NP-integrated stem cell therapy in clinical applications, NP behavior in xenogenic-free stem cell cultures needs to be understood in detail. Mesoporous silica nanoparticles (MSN) are profusely used in biomedical applications and have also shown great potential in stem cell therapies. One of the strategies to make them compatible with stem cell therapy is to alter their surface functionalization. In line with this notion, the main interest of the present investigation was to study the impact of human serum albumin (HSA) association with differently surface-modified MSN, and rationalize the MSN utilization in xenogenic-free stem cell culture by employing C2C12 myogenic progenitor cells as a model system. Our results revealed that HSA coating on differently surface-modified MSN is a promising strategy to improve the colloidal stability of MSN, stem cell viability, and imparts no adverse effects on the differentiation of stem cells.
KW - Cellular uptake
KW - Copolymer coatings
KW - Human serum albumin binding
KW - Mesoporous silica nanoparticles
KW - Stem cell therapy
UR - http://www.scopus.com/inward/record.url?scp=85084940304&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2020.124945
DO - 10.1016/j.colsurfa.2020.124945
M3 - Article
AN - SCOPUS:85084940304
SN - 0927-7757
VL - 600
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
M1 - 124945
ER -