Protein synthesis is required for stabilization of hsp70 mRNA upon exposure to both hydrostatic pressurization and elevated temperature

Kaarniranta, Holmberg, Helminen, John Eriksson, Lea Sistonen, Lammi

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    Sammanfattning

    We have recently described that in chondrocytic cells high hydrostatic pressure (HP) causes a heat shock response via mRNA stabilization without a transcriptional activation of the hsp70 gene. In this study, we investigated whether this exceptional regulatory mechanism occurs more generally in different types of cells. Indeed, hsp70 mRNA and protein accumulated in HeLa, HaCat and MG-63 cells under 30 MPa HP, without DNA-binding of heat shock transcription factor 1 (HSF1) to the heat shock element of the hsp70 gene or formation of nuclear HSF1 granules, revealing a lack of transcriptional activation. Moreover, we observed that protein synthesis is needed for mRNA stabilization. Thus, high HP offers a model to study the mechanisms of hsp70 mRNA stabilization without HSF1-mediated induction of the heat shock gene response.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)283–286
    TidskriftFEBS Letters
    Volym475
    Utgåva3
    StatusPublicerad - 2000
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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