Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

ME Sandberg; D Schellmann; G Brunhofer; T Erker; I Busygin; Reko Leino; PM Vuorela; A Fallarero

doi:10.1016/j.mimet.2009.04.014

Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

ME Sandberg
, D Schellmann
, G Brunhofer
, T Erker
, I Busygin
, Reko Leino
, PM Vuorela
, A Fallarero

Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review

85 Citeringar (Scopus)

Sammanfattning

Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.

Originalspråk	Odefinierat/okänt
Sidor (från-till)	104–106
Antal sidor	3
Tidskrift	Journal of Microbiological Methods
Volym	78
Nummer	1
DOI	https://doi.org/10.1016/j.mimet.2009.04.014
Status	Publicerad - 2009
MoE-publikationstyp	A1 Tidskriftsartikel-refererad

Nyckelord

Biofilms
Resazurin
Screening
Staphylococcus aureus

Åtkomst av dokument

10.1016/j.mimet.2009.04.014

Citera det här

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title = "Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay",

abstract = "Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.",

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author = "ME Sandberg and D Schellmann and G Brunhofer and T Erker and I Busygin and Reko Leino and PM Vuorela and A Fallarero",

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T1 - Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

AU - Sandberg, ME

AU - Schellmann, D

AU - Brunhofer, G

AU - Erker, T

AU - Busygin, I

AU - Leino, Reko

AU - Vuorela, PM

AU - Fallarero, A

PY - 2009

Y1 - 2009

N2 - Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.

AB - Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.

KW - Biofilms

KW - Resazurin

KW - Screening

KW - Staphylococcus aureus

KW - Biofilms

KW - Resazurin

KW - Screening

KW - Staphylococcus aureus

KW - Biofilms

KW - Resazurin

KW - Screening

KW - Staphylococcus aureus

U2 - 10.1016/j.mimet.2009.04.014

DO - 10.1016/j.mimet.2009.04.014

M3 - Artikel

SN - 0167-7012

VL - 78

SP - 104

EP - 106

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -