Prognostic value of using bee and hive debris samples for the detection of American foulbrood disease in honey bee colonies

Eva Forsgren, Ane Laugen

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    24 Citeringar (Scopus)

    Sammanfattning

    The objective of this study was to compare how well the detection of Paenibacillus larvae in samples of live bees or in accumulated winter debris collected from the bottom of beehives relates to symptoms of American foulbrood in honey bee colonies. Fifty-eight colonies in one commercial beekeeping operation were inspected for disease symptoms and assayed for P. larvae using culture-based techniques and PCR. The results show that culture-based techniques are more accurate than recently published PCR methods for detecting the bacterium in clinically diseased colonies, and that the prognostic value of bacterial colony counts from bee samples is superior to colony counts from debris. However, if the objective is to monitor the prevalence of the bacterium irrespective of disease symptoms, the preferable method is PCR analysis of accumulated winter hive debris.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)10–20
    Antal sidor11
    TidskriftApidologie
    Volym45
    Nummer1
    DOI
    StatusPublicerad - 2014
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Nyckelord

    • American foulbrood
    • culture
    • logistic regression
    • Paenibacillus larvae
    • PCR

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