TY - JOUR
T1 - Potential Probiotic Characters of Lactobacillus and Enterococcus Strains Isolated from Traditional Dadih Fermented Milk Against Pathogen Intestinal Colonization
AU - Collado, M. Carmen
AU - Surono, Ingrid S.
AU - Meriluoto, Jussi
AU - Salminen, Seppo
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2007/3
Y1 - 2007/3
N2 - Traditional fermented buffalo milk in Indonesia (dadih) has been believed to have a beneficial impact on human health, which could be related to the properties of the lactic acid bacteria (LAB) involved in its fermentation process. In previous studies, it was discovered that strains of dadih lactic isolates possessed some beneficial properties in vitro. In the present study, the adhesion capacity of specific LAB isolates from dadih to intestinal mucus was analyzed. Further, the ability to inhibit model human pathogens and displace them from mucus was assessed. The adhesion of tested LAB strains was strain-dependent and varied from 1.4 to 9.8%. The most adhesive Lactobacillus plantarum strain was IS-10506, with 9.8% adhesion. The competition assay between dadih LAB isolates and pathogens showed that a 2-h preincubation with L. plantarum at 37°C significantly reduced pathogen adhesion to mucus. All tested LAB strains displaced and inhibited pathogen adhesion, but the results were strain-specific and dependent on time and pathogen strains. In general, L. plantarum IS-10506 showed the best ability against pathogen adhesion.
AB - Traditional fermented buffalo milk in Indonesia (dadih) has been believed to have a beneficial impact on human health, which could be related to the properties of the lactic acid bacteria (LAB) involved in its fermentation process. In previous studies, it was discovered that strains of dadih lactic isolates possessed some beneficial properties in vitro. In the present study, the adhesion capacity of specific LAB isolates from dadih to intestinal mucus was analyzed. Further, the ability to inhibit model human pathogens and displace them from mucus was assessed. The adhesion of tested LAB strains was strain-dependent and varied from 1.4 to 9.8%. The most adhesive Lactobacillus plantarum strain was IS-10506, with 9.8% adhesion. The competition assay between dadih LAB isolates and pathogens showed that a 2-h preincubation with L. plantarum at 37°C significantly reduced pathogen adhesion to mucus. All tested LAB strains displaced and inhibited pathogen adhesion, but the results were strain-specific and dependent on time and pathogen strains. In general, L. plantarum IS-10506 showed the best ability against pathogen adhesion.
UR - http://www.scopus.com/inward/record.url?scp=33947434068&partnerID=8YFLogxK
U2 - 10.4315/0362-028X-70.3.700
DO - 10.4315/0362-028X-70.3.700
M3 - Article
C2 - 17388062
AN - SCOPUS:33947434068
SN - 0362-028X
VL - 70
SP - 700
EP - 705
JO - Journal of Food Protection
JF - Journal of Food Protection
IS - 3
ER -