Oxidation of lignans and lignin model compounds by laccase in aqueous solvent systems

Maija-Liisa Mattinen, Pekka Maijala, Paula Nousiainen, Annika Smeds, Jussi Kontro, Jussi Sipilä, Tarja Tamminen, Stefan Willför, Liisa Viikari

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    34 Citeringar (Scopus)


    The stability and activity of the low redox potential Melanocarpus albomyces laccase (MaL) in various aqueous organic (acetone, ethanol, propylene glycol, diethylene glycol monomethyl ether) solvent systems was studied spectrophotometrically using 2,6-dimethoxyphenol (2,6-DMP) as substrate. In addition, reactivity of the enzyme with two lignans; matairesinol (MR) and 7-hydroxymatairesinol (HMR), was examined by oxygen consumption measurements in the most potential aqueous organic solvent systems. Polymerization of the lignans by MaL was verified by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and size exclusion chromatography (SEC). Polymerization of the higher molecular weight lignin model compound, dehydrogenation polymers (DHPs), was studied by SEC. The solubilities of industrial softwood and hardwood kraft lignins were evaluated as parameters for investigation of enzymatic modification in aqueous organic solvent systems. The functioning of MaL in different aqueous organic media was excellent. Propylene glycol and diethylene glycol monomethyl ether were better solvents than ethanol or acetone in enzymatic oxidations. Even though they were the best solvents for enzyme oxidation, ethanol and propylene glycol were selected for further tests because of their different physicochemical properties. The results obtained in this study for the use of laccase-catalysed reactions in organic solvents to improve the efficiency of lignin oxidation may be exploited in several applications and areas in which the solubility of the reactants or products is a limiting factor.
    Sidor (från-till)122–129
    Antal sidor8
    TidskriftJournal of Molecular Catalysis B: Enzymatic
    StatusPublicerad - 2011
    MoE-publikationstypA1 Tidskriftsartikel-refererad


    • Dehydrogenation polymers
    • Laccase
    • Lignin
    • Melanocarpus albomyces
    • Reactivity
    • Solvent

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