Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

Camilo Guzman, Maja Solman, Daniel Abankwa

Forskningsoutput: Kapitel i bok/konferenshandlingKapitelVetenskapligPeer review

4 Citeringar (Scopus)

Sammanfattning

Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.
OriginalspråkOdefinierat/okänt
Titel på värdpublikationRas Signalling
RedaktörerLorenza Trabalzini, Saverio Francesco Retta
FörlagHumana Press
Sidor307–326
ISBN (elektroniskt)978-1-62703-791-4
ISBN (tryckt)978-1-62703-790-7
DOI
StatusPublicerad - 2014
MoE-publikationstypA3 Del av bok eller annan forskningsbok

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