Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

Camilo Guzman; Maja Solman; Daniel Abankwa

doi:10.1007/978-1-62703-791-4_20

Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

Camilo Guzman, Maja Solman, Daniel Abankwa

Åbo biovetenskapscentrum

Forskningsoutput: Kapitel i bok/konferenshandling › Kapitel › Vetenskaplig › Peer review

5 Citeringar (Scopus)

Sammanfattning

Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.

Originalspråk	Odefinierat/okänt
Titel på värdpublikation	Ras Signalling
Redaktörer	Lorenza Trabalzini, Saverio Francesco Retta
Förlag	Humana Press
Sidor	307–326
ISBN (elektroniskt)	978-1-62703-791-4
ISBN (tryckt)	978-1-62703-790-7
DOI	https://doi.org/10.1007/978-1-62703-791-4_20
Status	Publicerad - 2014
MoE-publikationstyp	A3 Del av bok eller annan forskningsbok

Åtkomst av dokument

10.1007/978-1-62703-791-4_20

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24470034&retmode=ref&cmd=prlinks

Citera det här

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abstract = "Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.",

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AU - Abankwa, Daniel

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AB - Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.

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