Molecular features of phospholipids that affect glycolipid transfer protein-mediated galactosylceramide transfer between vesicles

M Nylund, Matti Kjellberg, JG Molotkovsky, HS Byun, R Bittman, Peter Mattjus

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    20 Citeringar (Scopus)

    Sammanfattning

    The glycolipid transfer protein (GLTP)-mediated movement of galactosylceramide from model membrane donor vesicles to acceptor vesicles is sensitive to the membrane environment surrounding the glycolipid. GLTP can catalyze the transfer of a fluorescently labeled GSL, anthrylvinyl-galactosylceramide (AV-GalCer), from vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-pbosphocholine and dipalmitoylphosphatidylcholine matrices, but not from vesicles prepared from N-palmitoylsphingomyelin, regardless of the cholesterol content of the vesicles. In this study, we have examined the structural features of sphingomyelin (SM) that are responsible for its inhibition of the rate of GLTP-catalyzed transfer of AV-GalCer. The rate of glycolipid transfer was enhanced when the N-palmitoyl chain of SM was replaced with an N-oleoyl chain. Analogs of N-palmitoyl-SM in which the 4,5-double bond of the long-chain base is reduced or the 3-hydroxy group is removed did not inhibit GLTP-catalyzed transfer of AV-GalCer. When the donor vesicles were prepared with phosphatidyleholines or ether-linked phosphatidylcholine analogs, the transfer rates of AV-GalCer increased with increasing degree of unsaturation. The rate of AV-GalCer transfer was strongly dependent on the unsaturation degree of the acyl and/or alkyl chains. For ester-linked PCs, the transfer rate increased in the order DPPC < POPC < DOPC, which have 0, 1, and 2 cis double bonds, respectively.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)807–812
    Antal sidor6
    TidskriftBBA - Biomembranes
    Volym1758
    Utgåva6
    DOI
    StatusPublicerad - 2006
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Nyckelord

    • cholesterol
    • ether lipid
    • fluorescence
    • glycolipid transfer protein
    • glycosphingolipid
    • phosphatidylcholine
    • sphingomyelin
    • sphingomyelin analog

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