Mapping the Localization of Proteins Within Filopodia Using FiloMap

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Sammanfattning

Filopodia are fingerlike membrane protrusions extended by cells to sense their environment. Filopodia are widely used by migrating cells in vivo and directly contribute to several physiological processes and diseases. Due to the essential roles of filopodia in sensing the extracellular environment, there is a need to characterize their composition and ultrastructure further. This chapter highlights FiloMap, an image analysis pipeline that utilizes Fiji and R to map the localization of proteins within filopodia from microscopy images. I provide step-by-step protocols on (a) setting up FiloMap in Fiji and R, (b) extracting line intensity profiles from filopodia stainings in Fiji, (c) further analyzing line intensity profiles in R, and (d) creating filopodia maps to compare the localization of multiple proteins within filopodia. Notably, while FiloMap was written to analyze filopodia, the analysis pipeline described here can also analyze and compile any line intensity profiles.

OriginalspråkEngelska
Titel på värdpublikationMethods in Molecular Biology
RedaktörerCoert Margadant
FörlagHumana press
Sidor51-61
Antal sidor11
Volym2608
ISBN (elektroniskt)978-1-0716-2887-4
ISBN (tryckt)978-1-0716-2886-7
DOI
StatusPublicerad - 19 jan. 2023
MoE-publikationstypA3 Del av bok eller annan forskningsbok

Publikationsserier

NamnMethods in Molecular Biology
Volym2608
ISSN (tryckt)1064-3745
ISSN (elektroniskt)1940-6029

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