BACKGROUND: A systematic investigation of mutual interference between a hydrogenation catalyst, Pd/Al2O3, and an immobilized lipase in a one-pot synthesis of R-1-phenyl ethyl acetate at 70 °C has been undertaken. This paper reports the kinetic modeling of lipase-mediated chemo-bio cascade synthesis of R-1-phenyl ethyl acetate starting from acetophenone. RESULTS: The kinetic results revealed that these catalysts were not acting independently but in concert. A mechanism which predicts the experimental observations for this reaction is proposed. CONCLUSION: The parameters of the kinetic model, which are in good agreement with the experimental data, were estimated through numerical data fitting. The reliability of the estimated parameters was analyzed using the Markov Chain Monte Carlo (MCMC) method.