Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes

Chow, Weis, Kass, Holmström, John Eriksson, Orrenius

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    138 Citeringar (Scopus)

    Sammanfattning

    The mechanism of Fas antigen-mediated apoptosis is at present unclear. We show here that the 100,000 x g supernatant from cell lysates prepared from anti-Fas-stimulated JUR-KAT T cells, induces chromatin fragmentation in isolated nuclei with concomitant morphological changes typically seen in apoptosis. The formation of this apoptotic nuclei promoting activity (ANPA) in JURKAT T cells after Fas antigen ligation was blocked by the serine protease inhibitors, TPCK and DCI, and by the interleukin 1-beta-converting enzyme inhibitor, VAD-FMK. In addition, chromatin degradation and morphological changes mediated by the ANPA in isolated nuclei were inhibited by TPCK, but not by DCI or VAD-FMK. These results suggest that Fas-mediated apoptosis in T cells involves the activation of a cascade of proteases.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)134–138
    TidskriftFEBS Letters
    Volym364
    Nummer2
    StatusPublicerad - 1995
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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