Cell surface properties of dadih lactic acid bacteria strains were studied for adhesion to hydrocarbons (BATH) and aggregation abilities. Autoaggregation correlates with adhesion, which is a prerequisite for colonization and infection of the gastrointestinal tract by many pathogens, whereas coaggregation has been related to the ability to interact closely with pathogens. The results demonstrated significant differences in cell surface properties among the tested natural lactic acid bacteria food strains. Hydrophobicity increased when the cells were heat inactivated. All strains showed aggregation abilities with the pathogen strains tested, but the coaggregation properties were strain-specific. Our results indicate that the ability to autoaggregate, together with cell surface hydrophobicity and coaggregation abilities with pathogen strains, can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human or animal use. This study suggest the importance to identify and characterize bacterial cell-wall properties to understand their role in adhesion to hydrocarbons, autoaggregation and relation to coaggregation mechanisms, and also the relevance to future probiotic food development from natural strains.