Filopodia Quantification Using FiloQuant.

Guillaume Jacquemet, Hamidi, Ivaska

Forskningsoutput: Kapitel i bok/konferenshandlingKapitelVetenskapligPeer review

3 Citeringar (Scopus)
3 Nedladdningar (Pure)

Sammanfattning

Filopodia are fingerlike membrane protrusions that are extended by cells in vitro and in vivo. Due to important roles in sensing the extracellular microenvironment, filopodia and filopodia-like protrusions have been implicated in numerous biological processes including epithelial sheet zippering in development and wound healing and in cancer progression. Recently, there has been an explosion in the number of software available to analyze specific features of cell protrusions with the aim of gaining mechanistic insights into the action of filopodia and filopodia-like structures. In this methods chapter, we highlight an open-access software called FiloQuant that has been developed to specifically quantify the length, density, and dynamics of filopodia and filopodia-like structures from in vitro and in vivo generated samples. We provide step-by-step protocols on (i) how to install FiloQuant in the ImageJ platform (Fiji), (ii) how to quantify filopodia and filopodia-like protrusions from single images using FiloQuant, and (iii) how to track filopodial protrusions from live-cell imaging experiments using FiloQuant and TrackMate.
OriginalspråkOdefinierat/okänt
Titel på gästpublikationComputer Optimized Microscopy
RedaktörerElena Rebollo, Manel Bosch
FörlagHumana Press
Sidor359–373
ISBN (tryckt)978-1-4939-9685-8
DOI
StatusPublicerad - 2019
MoE-publikationstypA3 Del av bok eller annan forskningsbok

Citera det här