@article{6ede6b5448374a3c995484d7951e0555,
title = "Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery",
abstract = "Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.",
keywords = "adenovirus, cancer therapy, DNA delivery, Extracellular vesicles",
author = "Heikki Saari and Tiia Turunen and Andres L{\~o}hmus and Mikko Turunen and Matti Jalasvuori and Butcher, {Sarah J.} and Seppo Yl{\"a}-Herttuala and Tapani Viitala and Vincenzo Cerullo and Siljander, {Pia R.M.} and Marjo Yliperttula",
note = "Funding Information: We thank Benita L{\"o}flund, Pasi Laurinm{\"a}ki (University of Helsinki), Instruct-FI, the Biocenter Finland National cryo-electron microscopy units, the Electron Microscopy Unit of the Institute of Biotechnology, the Instruct-HiLIFE Biocomplex unit, member of Biocenter Finland and Instruct-FI and the EV-core, for providing technical assistance and facilities to carry out the work. We would also like to thank the Academy of Finland, Alfred Kordelin foundation and Emil Aaltonen foundation for funding this project. Funding Information: This work was supported by the Academy of Finland [287089, 292275, 268376, 314985, 328601, 315409]; Alfred Kordelinin S??ti? [160385]; Emil Aaltosen S??ti? [170223]; Suomen Kulttuurirahasto [2018]. We thank Benita L?flund, Pasi Laurinm?ki (University of Helsinki), Instruct-FI, the Biocenter Finland National cryo-electron microscopy units, the Electron Microscopy Unit of the Institute of Biotechnology, the Instruct-HiLIFE Biocomplex unit, member of Biocenter Finland and Instruct-FI and the EV-core, for providing technical assistance and facilities to carry out the work. We would also like to thank the Academy of Finland, Alfred Kordelin foundation and Emil Aaltonen foundation for funding this project. Publisher Copyright: {\textcopyright} 2020, {\textcopyright} 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.",
year = "2020",
month = jan,
day = "1",
doi = "10.1080/20013078.2020.1747206",
language = "English",
volume = "9",
journal = "Journal of extracellular vesicles",
issn = "2001-3078",
publisher = "Taylor & Francis",
number = "1",
}