Exosomal vimentin from adipocyte progenitors protects fibroblasts against osmotic stress and inhibits apoptosis to enhance wound healing

Sepideh Parvanian; Hualian Zha; Dandan Su; Lifang Xi; Yaming Jiu; Hongbo Chen; John E. Eriksson; Fang Cheng

doi:10.3390/ijms22094678

Exosomal vimentin from adipocyte progenitors protects fibroblasts against osmotic stress and inhibits apoptosis to enhance wound healing

Sepideh Parvanian
, Hualian Zha
, Dandan Su
, Lifang Xi
, Yaming Jiu
, Hongbo Chen
, John E. Eriksson
, Fang Cheng^*

^*Korresponderande författare för detta arbete

Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review

28 Citeringar (Scopus)

166 Nedladdningar (Pure)

Sammanfattning

Mechanical stress following injury regulates the quality and speed of wound healing. Improper mechanotransduction can lead to impaired wound healing and scar formation. Vimentin intermediate filaments control fibroblasts’ response to mechanical stress and lack of vimentin makes cells significantly vulnerable to environmental stress. We previously reported the involvement of exosomal vimentin in mediating wound healing. Here we performed in vitro and in vivo experiments to explore the effect of wide-type and vimentin knockout exosomes in accelerating wound healing under osmotic stress condition. Our results showed that osmotic stress increases the size and enhances the release of exosomes. Furthermore, our findings revealed that exosomal vimentin enhances wound healing by protecting fibroblasts against osmotic stress and inhibiting stress-induced apoptosis. These data suggest that exosomes could be considered either as a stress modifier to restore the osmotic balance or as a conveyer of stress to induce osmotic stress-driven conditions.

Originalspråk	Engelska
Artikelnummer	4678
Tidskrift	International Journal of Molecular Sciences
Volym	22
Nummer	9
DOI	https://doi.org/10.3390/ijms22094678
Status	Publicerad - 1 maj 2021
MoE-publikationstyp	A1 Tidskriftsartikel-refererad

Finansiering

This project funded by Sigrid Juselius Stiftelse and ?bo Akademi University, the National Natural Science Foundation of China (81702750, 81670141, 81970145, 81502663, 31970660); Science, Technology & Innovation Commission of Shenzhen Municipality (JCY20170818164756460, JCYJ20180307154700308, JCYJ20170818163844015); The Social Development Foundation of Jiangsu Province (BE2018691); Natural Science Foundation of Shanghai (19ZR1463000); ?100 talents program? from the Chinese Academy of Sciences and Shanghai Talent Development Funding. Imaging studies have been done at Turku Cell Imaging. Vim?/?APCs were obtained from Genome Editing Core, Turku Bioscience Centre. TEM imaging and NTA analysis were performed by the EV Core and Electron Microscopy Unit of the Institute of Biotechnology, University of Helsinki. Funding: This project funded by Sigrid Juselius Stiftelse and Åbo Akademi University, the National Natural Science Foundation of China (81702750, 81670141, 81970145, 81502663, 31970660); Science, Technology & Innovation Commission of Shenzhen Municipality (JCY20170818164756460, JCYJ20180307154700308, JCYJ20170818163844015); The Social Development Foundation of Jiangsu Province (BE2018691); Natural Science Foundation of Shanghai (19ZR1463000); “100 talents program” from the Chinese Academy of Sciences and Shanghai Talent Development Funding. Imaging studies have been done at Turku Cell Imaging.

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10.3390/ijms22094678

ijms-22-04678Slutlig publicerad version, 4,78 MBLicens: CC BY

https://urn.fi/URN:NBN:fi-fe2022021418860

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@article{d03a80fcb3424bcd8e6e1cefc343918e,

title = "Exosomal vimentin from adipocyte progenitors protects fibroblasts against osmotic stress and inhibits apoptosis to enhance wound healing",

abstract = "Mechanical stress following injury regulates the quality and speed of wound healing. Improper mechanotransduction can lead to impaired wound healing and scar formation. Vimentin intermediate filaments control fibroblasts{\textquoteright} response to mechanical stress and lack of vimentin makes cells significantly vulnerable to environmental stress. We previously reported the involvement of exosomal vimentin in mediating wound healing. Here we performed in vitro and in vivo experiments to explore the effect of wide-type and vimentin knockout exosomes in accelerating wound healing under osmotic stress condition. Our results showed that osmotic stress increases the size and enhances the release of exosomes. Furthermore, our findings revealed that exosomal vimentin enhances wound healing by protecting fibroblasts against osmotic stress and inhibiting stress-induced apoptosis. These data suggest that exosomes could be considered either as a stress modifier to restore the osmotic balance or as a conveyer of stress to induce osmotic stress-driven conditions.",

keywords = "Exosome, Mechanical stress, Osmotic stress, Vimentin, Wound healing",

author = "Sepideh Parvanian and Hualian Zha and Dandan Su and Lifang Xi and Yaming Jiu and Hongbo Chen and Eriksson, \{John E.\} and Fang Cheng",

note = "Funding Information: This project funded by Sigrid Juselius Stiftelse and ?bo Akademi University, the National Natural Science Foundation of China (81702750, 81670141, 81970145, 81502663, 31970660); Science, Technology \& Innovation Commission of Shenzhen Municipality (JCY20170818164756460, JCYJ20180307154700308, JCYJ20170818163844015); The Social Development Foundation of Jiangsu Province (BE2018691); Natural Science Foundation of Shanghai (19ZR1463000); ?100 talents program? from the Chinese Academy of Sciences and Shanghai Talent Development Funding. Imaging studies have been done at Turku Cell Imaging. Vim?/?APCs were obtained from Genome Editing Core, Turku Bioscience Centre. TEM imaging and NTA analysis were performed by the EV Core and Electron Microscopy Unit of the Institute of Biotechnology, University of Helsinki. Funding Information: Funding: This project funded by Sigrid Juselius Stiftelse and {\AA}bo Akademi University, the National Natural Science Foundation of China (81702750, 81670141, 81970145, 81502663, 31970660); Science, Technology \& Innovation Commission of Shenzhen Municipality (JCY20170818164756460, JCYJ20180307154700308, JCYJ20170818163844015); The Social Development Foundation of Jiangsu Province (BE2018691); Natural Science Foundation of Shanghai (19ZR1463000); “100 talents program” from the Chinese Academy of Sciences and Shanghai Talent Development Funding. Imaging studies have been done at Turku Cell Imaging. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = may,

day = "1",

doi = "10.3390/ijms22094678",

language = "English",

volume = "22",

journal = "International Journal of Molecular Sciences",

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T1 - Exosomal vimentin from adipocyte progenitors protects fibroblasts against osmotic stress and inhibits apoptosis to enhance wound healing

AU - Parvanian, Sepideh

AU - Zha, Hualian

AU - Su, Dandan

AU - Xi, Lifang

AU - Jiu, Yaming

AU - Chen, Hongbo

AU - Eriksson, John E.

AU - Cheng, Fang

N1 - Funding Information: This project funded by Sigrid Juselius Stiftelse and ?bo Akademi University, the National Natural Science Foundation of China (81702750, 81670141, 81970145, 81502663, 31970660); Science, Technology & Innovation Commission of Shenzhen Municipality (JCY20170818164756460, JCYJ20180307154700308, JCYJ20170818163844015); The Social Development Foundation of Jiangsu Province (BE2018691); Natural Science Foundation of Shanghai (19ZR1463000); ?100 talents program? from the Chinese Academy of Sciences and Shanghai Talent Development Funding. Imaging studies have been done at Turku Cell Imaging. Vim?/?APCs were obtained from Genome Editing Core, Turku Bioscience Centre. TEM imaging and NTA analysis were performed by the EV Core and Electron Microscopy Unit of the Institute of Biotechnology, University of Helsinki. Funding Information: Funding: This project funded by Sigrid Juselius Stiftelse and Åbo Akademi University, the National Natural Science Foundation of China (81702750, 81670141, 81970145, 81502663, 31970660); Science, Technology & Innovation Commission of Shenzhen Municipality (JCY20170818164756460, JCYJ20180307154700308, JCYJ20170818163844015); The Social Development Foundation of Jiangsu Province (BE2018691); Natural Science Foundation of Shanghai (19ZR1463000); “100 talents program” from the Chinese Academy of Sciences and Shanghai Talent Development Funding. Imaging studies have been done at Turku Cell Imaging. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Mechanical stress following injury regulates the quality and speed of wound healing. Improper mechanotransduction can lead to impaired wound healing and scar formation. Vimentin intermediate filaments control fibroblasts’ response to mechanical stress and lack of vimentin makes cells significantly vulnerable to environmental stress. We previously reported the involvement of exosomal vimentin in mediating wound healing. Here we performed in vitro and in vivo experiments to explore the effect of wide-type and vimentin knockout exosomes in accelerating wound healing under osmotic stress condition. Our results showed that osmotic stress increases the size and enhances the release of exosomes. Furthermore, our findings revealed that exosomal vimentin enhances wound healing by protecting fibroblasts against osmotic stress and inhibiting stress-induced apoptosis. These data suggest that exosomes could be considered either as a stress modifier to restore the osmotic balance or as a conveyer of stress to induce osmotic stress-driven conditions.

AB - Mechanical stress following injury regulates the quality and speed of wound healing. Improper mechanotransduction can lead to impaired wound healing and scar formation. Vimentin intermediate filaments control fibroblasts’ response to mechanical stress and lack of vimentin makes cells significantly vulnerable to environmental stress. We previously reported the involvement of exosomal vimentin in mediating wound healing. Here we performed in vitro and in vivo experiments to explore the effect of wide-type and vimentin knockout exosomes in accelerating wound healing under osmotic stress condition. Our results showed that osmotic stress increases the size and enhances the release of exosomes. Furthermore, our findings revealed that exosomal vimentin enhances wound healing by protecting fibroblasts against osmotic stress and inhibiting stress-induced apoptosis. These data suggest that exosomes could be considered either as a stress modifier to restore the osmotic balance or as a conveyer of stress to induce osmotic stress-driven conditions.

KW - Exosome

KW - Mechanical stress

KW - Osmotic stress

KW - Vimentin

KW - Wound healing

UR - https://www.scopus.com/pages/publications/85104825282

U2 - 10.3390/ijms22094678

DO - 10.3390/ijms22094678

M3 - Article

C2 - 33925176

AN - SCOPUS:85104825282

SN - 1661-6596

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 9

M1 - 4678

ER -

Exosomal vimentin from adipocyte progenitors protects fibroblasts against osmotic stress and inhibits apoptosis to enhance wound healing

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