Evaluation of Antibacterial and Anti-biofilm Activities of Cinchona Alkaloid Derivatives against Staphylococcus aureus

Malena Skogman, Janni Kujala, I Busygin, Reko Leino, Pia Vuorela, Adyary Fallarero

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Bacterial biofilms are resistant to most of the commonly available antibacterial chemotherapies. Thus, an enormous need exists to meet the demands of effective anti-biofilm therapy. In this study, a small library of cinchona alkaloids, including the naturally occurring compounds cinchonidine and cinchonine, as well as various synthetic derivatives and analogues was screened for antibacterial and anti-biofilm activity against the Staphylococcus aureus biofilm producing strain ATCC 25923. Two methods were used to evaluate activity against biofilms, namely crystal violet staining to measure biomass and resazurin assay to measure biofilms viability. Cinchonidine was found to be inactive, whereas a synthetic derivative, 11-triphenylsilyl-10,11-dihydrocinchonidine (11-TPSCD), was effective against planktonic bacteria as well as in preventing biofilm formation at low micromolar concentrations. Higher concentrations were required to eradicate mature biofilms.
OriginalspråkOdefinierat/okänt
Sidor (från-till)1173–1176
Antal sidor4
TidskriftNatural Product Communications
Volym7
Utgåva9
StatusPublicerad - 2012
MoE-publikationstypA1 Tidskriftsartikel-refererad

Nyckelord

  • Biofilms
  • ChemGPS-NP
  • Cinchona alkaloid
  • Fluorescence microscopy
  • Staphylococcus aureus
  • Antimicrobial

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