Enhanced protein adsorption and patterning on nanostructured latex-coated paper

Helka Juvonen, Anni Määttänen, Petri Ihalainen, Tapani Viitala, Jawad Sarfraz, Jouko Peltonen

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    11 Citeringar (Scopus)

    Sammanfattning

    Specific interactions of extracellular matrix proteins with cells and their adhesion to the substrate are important for cell growth. A nanopatterned latex-coated paper substrate previously shown to be an excellent substrate for cell adhesion and 2D growth was studied for directed immobilization of proteins. The nanostructured latex surface was formed by short-wavelength \{IR\} irradiation of a two-component latex coating consisting of a hydrophilic film-forming styrene butadiene acrylonitrile copolymer and hydrophobic polystyrene particles. The hydrophobic regions of the IR-treated latex coating showed strong adhesion of bovine serum albumin (cell repelling protein), fibronectin (cell adhesive protein) and streptavidin. Opposite to the IR-treated surface, fibronectin and streptavidin had a poor affinity toward the untreated pristine latex coating. Detailed characterization of the physicochemical surface properties of the latex-coated substrates revealed that the observed differences in protein affinity were mainly due to the presence or absence of the protein repelling polar and charged surface groups. The protein adsorption was assisted by hydrophobic (dehydration) interactions.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)261–269
    TidskriftColloids and Surfaces B: Biointerfaces
    Volym118
    Utgåva0
    DOI
    StatusPublicerad - 2014
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Nyckelord

    • protein adsorption
    • Patterning
    • coated paper
    • Latex

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