DNA rendering of polyhedral meshes at the nanoscale

Erik Benson, Abdulmelik Mohammed, Johan Gardell, Sergej Masich, Eugen Czeizler, Pekka Orponen, Bjorn Högberg

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    339 Citeringar (Scopus)

    Sammanfattning

    It was suggested more than thirty years ago that Watson–Crick base pairing might be used for the rational design of nanometre-scale structures from nucleic acids. Since then, and especially since the introduction of the origami technique, DNA nanotechnology has enabled increasingly more complex structures. But although general approaches for creating DNA origami polygonal meshes and design software are available, there are still important constraints arising from DNA geometry and sense/antisense pairing, necessitating some manual adjustment during the design process. Here we present a general method of folding arbitrary polygonal digital meshes in DNA that readily produces structures that would be very difficult to realize using previous approaches. The design process is highly automated, using a routeing algorithm based on graph theory and a relaxation simulation that traces scaffold strands through the target structures. Moreover, unlike conventional origami designs built from close-packed helices, our structures have a more open conformation with one helix per edge and are therefore stable under the ionic conditions usually used in biological assays.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)441–444
    TidskriftNature
    Volym523
    Utgåva7561
    DOI
    StatusPublicerad - 2015
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Nyckelord

    • self-assembly
    • Computer sciences
    • nanotechnology
    • DNA

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