TY - JOUR
T1 - Diatom-inspired skeletonisation of insulin - mechanistic insights into crystallisation and extracellular bioactivity
AU - Soto Véliz, Diosangeles
AU - Alam, Catharina
AU - Neitzel, Thiago
AU - Wyborski, Rebecca
AU - Rivero-Müller, Adolfo
AU - Alam, Parvez
N1 - paf
PY - 2015
Y1 - 2015
N2 - In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.
AB - In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.
U2 - 10.1016/j.colsurfb.2015.05.047
DO - 10.1016/j.colsurfb.2015.05.047
M3 - Artikel
SN - 0927-7765
VL - 133
SP - 140
EP - 147
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -