Diatom-inspired skeletonisation of insulin - mechanistic insights into crystallisation and extracellular bioactivity

Diosangeles Soto Véliz, Catharina Alam, Thiago Neitzel, Rebecca Wyborski, Adolfo Rivero-Müller, Parvez Alam

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    2 Citeringar (Scopus)

    Sammanfattning

    In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.
    OriginalspråkOdefinierat/okänt
    Sidor (från-till)140–147
    TidskriftColloids and Surfaces B: Biointerfaces
    Volym133
    DOI
    StatusPublicerad - 2015
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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