Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

Milla Riina Neffling; Emilie Lance; Jussi Meriluoto

doi:10.1016/j.envpol.2009.10.023

Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

Milla Riina Neffling^*
, Emilie Lance
, Jussi Meriluoto

^*Korresponderande författare för detta arbete

Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review

72 Citeringar (Scopus)

Sammanfattning

Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

Originalspråk	Engelska
Sidor (från-till)	948-952
Antal sidor	5
Tidskrift	Environmental Pollution
Volym	158
Nummer	3
DOI	https://doi.org/10.1016/j.envpol.2009.10.023
Status	Publicerad - mars 2010
MoE-publikationstyp	A1 Tidskriftsartikel-refererad

Finansiering

The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska understödsföreningen Liv och Hälsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study.

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title = "Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry",

abstract = "Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40\%), the extraction efficiency good (62-97\%), but the signal suppression effect from the matrix remained severe in our system (16-37\% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100\%, depending on the sample and the toxin variant and concentration.",

keywords = "Microcystins, MMPB method, Total contamination levels",

author = "Neffling, \{Milla Riina\} and Emilie Lance and Jussi Meriluoto",

note = "Funding Information: The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska underst{\"o}dsf{\"o}reningen Liv och H{\"a}lsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "2010",

month = mar,

doi = "10.1016/j.envpol.2009.10.023",

language = "English",

volume = "158",

pages = "948--952",

journal = "Environmental Pollution",

issn = "0269-7491",

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AU - Neffling, Milla Riina

AU - Lance, Emilie

AU - Meriluoto, Jussi

N1 - Funding Information: The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska understödsföreningen Liv och Hälsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 2010/3

Y1 - 2010/3

N2 - Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

AB - Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

KW - Microcystins

KW - MMPB method

KW - Total contamination levels

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DO - 10.1016/j.envpol.2009.10.023

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JO - Environmental Pollution

JF - Environmental Pollution

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Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

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