Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling

Ponnuswamy Mohanasundaram, Leila S Coelho-Rato, Mayank Kumar Modi, Marta Urbanska, Franziska Lautenschläger, Fang Cheng, John E Eriksson

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

11 Citeringar (Scopus)
41 Nedladdningar (Pure)

Sammanfattning

The nutrient-activated mTORC1 (mechanistic target of rapamycin kinase complex 1) signaling pathway determines cell size by controlling mRNA translation, ribosome biogenesis, protein synthesis, and autophagy. Here, we show that vimentin, a cytoskeletal intermediate filament protein that we have known to be important for wound healing and cancer progression, determines cell size through mTORC1 signaling, an effect that is also manifested at the organism level in mice. This vimentin-mediated regulation is manifested at all levels of mTOR downstream target activation and protein synthesis. We found that vimentin maintains normal cell size by supporting mTORC1 translocation and activation by regulating the activity of amino acid sensing Rag GTPase. We also show that vimentin inhibits the autophagic flux in the absence of growth factors and/or critical nutrients, demonstrating growth factor-independent inhibition of autophagy at the level of mTORC1. Our findings establish that vimentin couples cell size and autophagy through modulating Rag GTPase activity of the mTORC1 signaling pathway.

OriginalspråkEngelska
Artikelnummere3001737
Antal sidor21
TidskriftPLoS Biology
Volym20
Nummer9
DOI
StatusPublicerad - 13 sep. 2022
MoE-publikationstypA1 Tidskriftsartikel-refererad

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