Sammanfattning
This work focused on the development and characterization of engineered switchable bioelectronics, with an emphasis on studying
peptide conformational changes at electrode surfaces. Achieving ondemand control over peptide structures enables temporal signaling
and offers insights into biological pathways, such as the Notch signaling system.
In Paper I, we investigated peptide conformational changes using underwater contact angle measurements. Our findings demonstrated
that functionalized surfaces could be modulated by applying electrical potential, with changes effectively monitored via surface property
measurements.
Paper II aimed to observe surface changes and evaluate the efficacy of bioelectronic switches directly in complex media using a fully
electrical apparatus. Organic electrochemical transistors (OECTs) emerged as promising tools for this purpose. We optimized their
fabrication for enhanced performance and robustness through experimental design and stencil printing techniques.
Paper III addressed the limitations and challenges associated with OECTs, particularly their operational stability. To this end, we designed a test protocol and explored a novel fabrication approach using direct ink writing. Although the stable OECTs were applied to measure peptide conformational changes, the results were unexpected, prompting further inquiry.
In addition, we extended our investigation to include an extended gate MOSFET setup. This system provided an alternative approach to
studying the conformational changes of charged peptides, complementing the insights gained from OECT-based studies. The extended setup provided information about the possibility of a higher level of complexity than expected, which requires additional investigation.
We introduced novel characterization techniques for switchable bioelectronics, developed new fabrication methods for OECTs, and addressed key operational stability challenges. These works have extended our understanding of bioelectronics, provided insights into
peptide functionalization at electrode interfaces, and provided tools for studying them.
peptide conformational changes at electrode surfaces. Achieving ondemand control over peptide structures enables temporal signaling
and offers insights into biological pathways, such as the Notch signaling system.
In Paper I, we investigated peptide conformational changes using underwater contact angle measurements. Our findings demonstrated
that functionalized surfaces could be modulated by applying electrical potential, with changes effectively monitored via surface property
measurements.
Paper II aimed to observe surface changes and evaluate the efficacy of bioelectronic switches directly in complex media using a fully
electrical apparatus. Organic electrochemical transistors (OECTs) emerged as promising tools for this purpose. We optimized their
fabrication for enhanced performance and robustness through experimental design and stencil printing techniques.
Paper III addressed the limitations and challenges associated with OECTs, particularly their operational stability. To this end, we designed a test protocol and explored a novel fabrication approach using direct ink writing. Although the stable OECTs were applied to measure peptide conformational changes, the results were unexpected, prompting further inquiry.
In addition, we extended our investigation to include an extended gate MOSFET setup. This system provided an alternative approach to
studying the conformational changes of charged peptides, complementing the insights gained from OECT-based studies. The extended setup provided information about the possibility of a higher level of complexity than expected, which requires additional investigation.
We introduced novel characterization techniques for switchable bioelectronics, developed new fabrication methods for OECTs, and addressed key operational stability challenges. These works have extended our understanding of bioelectronics, provided insights into
peptide functionalization at electrode interfaces, and provided tools for studying them.
| Originalspråk | Engelska |
|---|---|
| Handledare |
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| Förlag | |
| Tryckta ISBN | 978-952-12-4471-1 , 978-952-12-4471-1 , 978-952-12-4471-1 , 978-952-12-4470-4 |
| Elektroniska ISBN | 978-952-12-4471-1 |
| Status | Publicerad - 2025 |
| MoE-publikationstyp | G5 Doktorsavhandling (artikel) |