Cells Respond to Mechanical Stress by Rapid Disassembly of Caveolae

B Sinha, D Köster, R Ruez, P Gonnord, M Bastiani, Daniel Abankwa, RV Stan, G Butler-Browne, B Vedie, L Johannes, N Morone, RG Parton, G Raposo, P Sens, C Lamaze, P Nassoy

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492 Citeringar (Scopus)

Sammanfattning

The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapid disappearance of caveolae, in a reduced caveolin/Cavin1 interaction, and in an increase of free caveolins at the plasma membrane. Tether-pulling force measurements in cells and in plasma membrane spheres demonstrate that caveola flattening and disassembly is the primary actin-and ATP-independent cell response that buffers membrane tension surges during mechanical stress. Conversely, stress release leads to complete caveola reassembly in an actin-and ATP-dependent process. The absence of a functional caveola reservoir in myotubes from muscular dystrophic patients enhanced membrane fragility under mechanical stress. Our findings support a new role for caveolae as a physiological membrane reservoir that quickly accommodates sudden and acute mechanical stresses.
OriginalspråkOdefinierat/okänt
Sidor (från-till)402–413
Antal sidor12
TidskriftCell
Volym144
Utgåva3
DOI
StatusPublicerad - 2011
MoE-publikationstypA1 Tidskriftsartikel-refererad

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