Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers

YM Jiu, J Lehtimaki, S Tojkander, Fang Cheng, H Jaalinoja, XN Liu, M Varjosalo, John Eriksson, P Lappalainen

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

70 Citeringar (Scopus)

Sammanfattning

The actin cytoskeleton and cytoplasmic intermediate filaments contribute to cell migration and morphogenesis, but the interplay between these two central cytoskeletal elements has remained elusive. Here, we find that specific actin stress fiber structures, transverse arcs, interact with vimentin intermediate filaments and promote their retrograde flow. Consequently, myosin-II-containing arcs are important for perinuclear localization of the vimentin network in cells. The vimentin network reciprocally restricts retrograde movement of arcs and hence controls the width of flat lamellum at the leading edge of the cell. Depletion of plectin recapitulates the vimentin organization phenotype of arc-deficient cells without affecting the integrity of vimentin filaments or stress fibers, demonstrating that this cytoskeletal crosslinker is required for productive interactions between vimentin and arcs. Collectively, our results reveal that plectin-mediated interplay between contractile actomyosin arcs and vimentin intermediate filaments controls the localization and dynamics of these two cytoskeletal systems and is consequently important for cell morphogenesis.
OriginalspråkOdefinierat/okänt
Sidor (från-till)1511–1518
Antal sidor8
TidskriftCell Reports
Volym11
Utgåva10
DOI
StatusPublicerad - 2015
MoE-publikationstypA1 Tidskriftsartikel-refererad

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