Activation of the MKK4-JNK pathway during erythroid differentiation of K562 cells is inhibited by the heat shock factor 2-beta isoform

Hietakangas, Elo, Rosenström, Eleanor Coffey, Kyriakis, John Eriksson, Lea Sistonen

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

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    In this study we report the activation of c-Jun N-terminal kinase (JNK) in human K562 erythroleukemia cells undergoing hemin-mediated erythroid differentiation, which occurs concomitantly with activation of heat shock factor 2 (HSF2) and leads to a simultaneous in vivo phosphorylation of c-Jun. The activation of JNK occurs through activation of mitogen-activated protein kinase kinase (MKK) 4 and not by activation of MKK7 or inhibition of JNK-directed phosphatases. We have previously shown that overexpression of the HSF2-beta isoform inhibits the activation of HSF2 upon hemin-induced erythroid differentiation. Here we demonstrate that HSF2-beta overexpression blocks the hemin-induced activation of the MKK4-JNK pathway, suggesting an erythroid lineage-specific JNK activation likely to be regulated by HSF2.
    Sidor (från-till)168–172
    TidskriftFEBS Letters
    StatusPublicerad - 2001
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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