TY - JOUR
T1 - A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
AU - Rosqvist, Emil
AU - Niemelä, Erik
AU - Frisk, Johan
AU - Öblom, Heidi
AU - Koppolu, Rajesh
AU - Abdelkader, Hend
AU - Soto Véliz, Diosangeles
AU - Mennillo, Marco
AU - Poonthuruthikudy Venu, Arun
AU - Ihalainen, Petri
AU - Aubert, Melanie
AU - Sandler, Niklas
AU - Wilen, Carl-Eric
AU - Toivakka, Martti
AU - Eriksson, John
AU - Österbacka, Ronald
AU - Peltonen, Jouko
N1 - fyke.
paf
fys.
tpk
Accepted manuscript, 12 mån embargo, Publisher rights policy
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PY - 2020
Y1 - 2020
N2 - A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
AB - A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
U2 - 10.1039/C9TB01958H
DO - 10.1039/C9TB01958H
M3 - Article
SN - 2050-750X
VL - 8
SP - 1146
EP - 1156
JO - Journal of Materials Chemistry. B
JF - Journal of Materials Chemistry. B
IS - 6
ER -