Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II

Koe, Tan, Max Lönnfors, Hur, Low, Zhang, Kanchanawong, Bankaitis, Wang

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

13 Sitaatiot (Scopus)

Abstrakti

A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs.
AlkuperäiskieliEi tiedossa
Sivut
JulkaisueLife
Vuosikerta7
DOI - pysyväislinkit
TilaJulkaistu - 2018
Julkaistu ulkoisestiKyllä
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

Viittausmuodot